A Gag peptide encompassing B- and T-cell epitopes of the caprine arthritis encephalitis virus functions as modular carrier peptide

Niederhauser, S.; Zahno, M.L.; Nenci, C.; Vogt, H.R.; Zanoni, R.; Peterhans, E.; Bertoni, G. (2009). A Gag peptide encompassing B- and T-cell epitopes of the caprine arthritis encephalitis virus functions as modular carrier peptide. Journal of immunological methods, 342(1-2), pp. 82-90. Amsterdam: Elsevier 10.1016/j.jim.2008.11.015

Full text not available from this repository. (Request a copy)

Short synthetic peptides are important tools in biomedical research permitting to generate hapten specific polyclonal sera for analytical purposes or functional studies. In this paper we provide proof of principle that a peptide located in a highly conserved portion of the Gag protein of the caprine arthritis encephalitis virus and carrying an immunodominant T helper cell epitope functions as an efficient carrier peptide, mediating a strong antibody response to a peptidic hapten encompassing a well-characterized B cell epitope of Env. The carrier and hapten peptides were collinearly synthesized permutating their molecular arrangement. While the antibody response to the hapten was similar for both constructs, the antibody response to a B cell epitope overlapping the T helper cell epitope of the Gag carrier peptide was considerably different. This permits a modular use of the carrier peptide to generate antibody directed exclusively to the hapten peptide or a strong humoral response to both carrier- and hapten-peptide. Finally, we have mapped the epitopes involved in this polarized antibody response and discussed the potential immunological implications.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology

UniBE Contributor:

Vogt, Hans-Rudolf; Zanoni, Reto Giacomo; Peterhans, Ernst and Bertoni, Giuseppe

ISSN:

0022-1759

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1016/j.jim.2008.11.015

Web of Science ID:

000264685300010

URI:

https://boris.unibe.ch/id/eprint/38311 (FactScience: 220994)

Actions (login required)

Edit item Edit item
Provide Feedback