Evaluation of a new commercial enzyme-linked immunosorbent assay for the detection of porcine antibodies against Trichinella spp

Frey, Caroline; Buholzer, P.; Beck, R.; Marinculic, A.; Raeber, A.J.; Gottstein, Bruno; Schuppers, M.E. (2009). Evaluation of a new commercial enzyme-linked immunosorbent assay for the detection of porcine antibodies against Trichinella spp. Journal of veterinary diagnostic investigation, 21(5), pp. 692-7. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063870902100516

[img] Text
Frey_evaluation of new comm ELISA_2009_JVDIpdf.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (304kB) | Request a copy

Trichinellosis is a zoonotic disease that is caused by the nematode Trichinella spp. Both European Union regulations and guidelines from the World Organization for Animal Health foresee the possibility of conducting serological surveillance for Trichinella spp. A newly developed commercial enzyme-linked immunosorbent assay (ELISA) was evaluated against 2 existing diagnostic techniques: an in-house ELISA and an in-house Western blot. A total of 875 Trichinella larva-negative samples of pigs and 93 Trichinella larva-positive samples of both naturally and experimentally infected pigs were included in the study. Bayesian modeling techniques were used to correct for the absence of a perfect reference test. The sensitivity and specificity of the commercial ELISA was 97.1-97.8% and 99.5-99.8%, respectively. Sensitivity analysis demonstrated high stability in the models. In a serological surveillance system, ELISA-positive samples should be tested by a confirmatory test. The Western blot is a suitable test for this purpose. With the use of the results of the models, the sensitivity and specificity of a test protocol in both ELISA and Western blot were 95.9% and 99.9%, respectively. The high sensitivity and specificity were achieved with a lower limit of detection than that of the routine artificial digestion test, suggesting that serological surveillance is a valuable alternative in surveillance for Trichinella spp. in pig production.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Frey, Caroline and Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture

ISSN:

1040-6387

Publisher:

American Association of Veterinary Laboratory Diagnosticians AAVLD

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

04 Feb 2016 10:16

Publisher DOI:

10.1177/104063870902100516

PubMed ID:

19737767

Web of Science ID:

000269963400016

BORIS DOI:

10.7892/boris.38330

URI:

https://boris.unibe.ch/id/eprint/38330 (FactScience: 221165)

Actions (login required)

Edit item Edit item
Provide Feedback