Validation of a Western Blot for the detection of anti-Trichinella spp. antibodies in domestic pigs

Frey, Caroline; Schuppers, M.E.; Nockler, K.; Marinculic, A.; Pozio, E.; Kihm, U.; Gottstein, Bruno (2009). Validation of a Western Blot for the detection of anti-Trichinella spp. antibodies in domestic pigs. Parasitology research, 104(6), pp. 1269-1277. Berlin: Springer-Verlag 10.1007/s00436-008-1321-9

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Trichinellosis is a zoonotic disease in humans caused by Trichinella spp. According to international regulations and guidelines, serological surveillance can be used to demonstrate the absence of Trichinella spp. in a defined domestic pig population. Most enzyme-linked immunosorbent assay (ELISA) tests presently available do not yield 100% specificity, and therefore, a complementary test is needed to confirm the diagnosis of any initial ELISA seropositivity. The goal of the present study was to evaluate the sensitivity and specificity of a Western Blot assay based on somatic Trichinella spiralis muscle stage (L1) antigen using Bayesian modeling techniques. A total of 295 meat juice and serum samples from pigs negative for Trichinella larvae by artificial digestion, including 74 potentially cross-reactive sera of pigs with other nematode infections, and 93 meat juice samples from pigs infected with Trichinella larvae were included in the study. The diagnostic sensitivity and specificity of the Western Blot were ranged from 95.8% to 96.0% and from 99.5% to 99.6%, respectively. A sensitivity analysis showed that the model outcomes were hardly influenced by changes in the prior distributions, providing a high confidence in the outcomes of the models. This validation study demonstrated that the Western Blot is a suitable method to confirm samples that reacted positively in an initial ELISA.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Frey, Caroline and Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture

ISSN:

0932-0113

Publisher:

Springer-Verlag

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

26 Jun 2018 14:40

Publisher DOI:

10.1007/s00436-008-1321-9

PubMed ID:

19130084

Web of Science ID:

000265442600004

BORIS DOI:

10.7892/boris.38333

URI:

https://boris.unibe.ch/id/eprint/38333 (FactScience: 221168)

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