Screening Swiss water bodies for potentially pathogenic free-living amoebae

Gianinazzi, C.; Schild, M.; Wuthrich, F.; Ben Nouir, N.; Fuchslin, H.P.; Schurch, N.; Gottstein, B.; Muller, N. (2009). Screening Swiss water bodies for potentially pathogenic free-living amoebae. Research in microbiology, 160(6), pp. 367-74. Amsterdam: Elsevier 10.1016/j.resmic.2009.06.007

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Free-ling amoebae (FLA) including Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia pedata, can cause opportunistic infections leading to severe brain pathologies. Human infections with pathogenic FLA have been increasingly documented in many countries. In Switzerland, thus far, the occurrence and distribution of potentially pathogenic FLA has not been investigated. Swiss water biotopes, including swimming pools, lakes, rivers and ponds, have now been screened for the presence of FLA, and assessment of their pathogenicity potential for a mammalian host has been undertaken. Thus, a total of 17 isolates were recovered by in vitro cultivation from these different aquatic sources. Characterization by sequence analysis of Acanthamoeba spp.-specific and 'FLA-specific PCR products amplified from 18s rDNA based on morphological traits, thermotolerance, and cytotoxicity towards murine fibroblasts yielded the following findings: Echinamoeba cf. exundans (3 isolates), Hartmannella spp. (3), Vannella spp. (4), Protacanthamoebica cf. bohemica (1), Acanthamoeba cf. castellanii (1) and Naegleria spp. (5). B. mandrillaris and N. fowleri did not range amongst these isolates. None of the isolates exhibited pronounced cytotoxicity and all failed to grow at 42 degrees C; therefore, they do not present any potential for CNS pathogenicity for humans.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gottstein, Bruno and Müller, Norbert

ISSN:

0923-2508

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 15:25

Last Modified:

28 Oct 2019 11:53

Publisher DOI:

10.1016/j.resmic.2009.06.007

PubMed ID:

19589386

Web of Science ID:

000270162300001

BORIS DOI:

10.7892/boris.38334

URI:

https://boris.unibe.ch/id/eprint/38334 (FactScience: 221169)

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