Potentially human pathogenic Acanthamoeba isolated from a heated indoor swimming pool in Switzerland

Gianinazzi, Christian; Schild, Marc; Wuthrich, F.; Müller, Norbert; Schurch, N.; Gottstein, Bruno (2009). Potentially human pathogenic Acanthamoeba isolated from a heated indoor swimming pool in Switzerland. Experimental parasitology, 121(2), pp. 180-186. Amsterdam: Elsevier 10.1016/j.exppara.2008.11.001

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Some free-living amoebae, including some species of the genus Acanthamoeba, can cause infections in humans and animals. These organisms are known to cause granulomatous amebic encephalitis (GAE) in predominantly immune-deficient persons. In the present study, we isolated a potentially human pathogenic Acanthamoeba isolate originating from a public heated indoor swimming pool in Switzerland. The amoebae, thermophilically preselected by culture at 37 degrees C, subsequently displayed a high thermotolerance, being able to grow at 42 degrees C, and a marked cytotoxicity, based on a co-culture system using the murine cell line L929. Intranasal infection of Rag2-immunodeficient mice resulted in the death of all animals within 24 days. Histopathology of brains and lungs revealed marked tissue necrosis and hemorrhagic lesions going along with massive proliferation of amoebae. PCR and sequence analysis, based on 18S rDNA, identified the agent as Acanthamoeba lenticulata. In summary, the present study reports on an Acanthamoeba isolate from a heated swimming pool suggestive of being potentially pathogenic to immunocompromised persons.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology

UniBE Contributor:

Gianinazzi, Christian; Schild, Marc; Müller, Norbert and Gottstein, Bruno

Subjects:

600 Technology > 630 Agriculture

ISSN:

0014-4894

Publisher:

Elsevier

Language:

English

Submitter:

Norbert Müller

Date Deposited:

04 Oct 2013 15:25

Last Modified:

17 Apr 2015 12:16

Publisher DOI:

10.1016/j.exppara.2008.11.001

PubMed ID:

19041307

Web of Science ID:

000262658900011

URI:

https://boris.unibe.ch/id/eprint/38335 (FactScience: 221170)

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