Protein changes and proteolytic degradation in red and white clover plants subjected to waterlogging

Stoychev, Veselin; Simova Stoilova, Lyudmila; Vaseva, Irina; Kostadinova, Anelia; Nenkova, Rosa; Feller, Urs; Demirevska, K. (2013). Protein changes and proteolytic degradation in red and white clover plants subjected to waterlogging. Acta Physiologiae Plantarum, 35(6), pp. 1925-1932. Berlin: Springer 10.1007/s11738-013-1231-z

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Red (Trifolium pratense L., cv. “Start”) and white clover varieties (Trifolium repens L., cv. “Debut” and cv. “Haifa”) were waterlogged for 14 days and subsequently recovered for the period of 21 days. Physiological and biochemical responses of the clover varieties were distinctive, which suggested different sensitivity toward flooding. The comparative study of morphological and biochemical parameters such as stem length, leaflet area, dry weight, protein content, protein pattern and proteolytic degradation revealed prominent changes under waterlogging conditions. Protease activity in the stressed plants increased significantly, especially in red clover cv. “Start”, which exhibited eightfold higher azocaseinolytic activity compared to the control. Changes in the protein profiles were detected by SDS-PAGE electrophoresis. The specific response of some proteins (Rubisco, Rubisco-binding protein, Rubisco activase, ClpA and ClpP protease subunits) toward the applied stress was assessed by immunoblotting. The results characterized the red clover cultivar “Start” as the most sensitive toward waterlogging, expressing reduced levels of Rubisco large and small subunits, high content of ClpP protease subunits and increased activity of protease isoforms.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
Graduate Schools > Graduate School of Climate Sciences

UniBE Contributor:

Simova Stoilova, Lyudmila and Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0137-5881

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Feb 2014 14:46

Last Modified:

05 Apr 2017 09:02

Publisher DOI:

10.1007/s11738-013-1231-z

Uncontrolled Keywords:

ClpA and ClpP protease subunits, Proteolysis, Rubisco, Red clover (Trifolium pratense L.), White clover (Trifolium repens L.), Waterlogging

BORIS DOI:

10.7892/boris.38660

URI:

https://boris.unibe.ch/id/eprint/38660

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