Characterization of choline uptake in Trypanosoma brucei procyclic and bloodstream forms

Macêdo, Juan P.; Schmidt, Remo S.; Mäser, Pascal; Rentsch, Doris; Vial, Henri J.; Sigel, Erwin; Bütikofer, Peter (2013). Characterization of choline uptake in Trypanosoma brucei procyclic and bloodstream forms. Molecular and biochemical parasitology, 190(1), pp. 16-22. Amsterdam: Elsevier 10.1016/j.molbiopara.2013.05.007

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Choline is an essential nutrient for eukaryotic cells, where it is used as precursor for the synthesis of choline-containing phospholipids, such as phosphatidylcholine (PC). According to published data, Trypanosoma brucei parasites are unable to take up choline from the environment but instead use lyso-phosphatidylcholine as precursor for choline lipid synthesis. We now show that T. brucei procyclic forms in culture readily incorporate [3H]-labeled choline into PC, indicating that trypanosomes express a transporter for choline at the plasma membrane. Characterization of the transport system in T. brucei procyclic and bloodstream forms shows that uptake of choline is independent of sodium and potassium ions and occurs with a Km in the low micromolar range. In addition, we demonstrate that choline uptake can be blocked by the known choline transport inhibitor, hemicholinium-3, and by synthetic choline analogs that have been established as anti-malarials. Together, our results show that T. brucei parasites express an uptake system for choline and that exogenous choline is used for PC synthesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Pereira de Macêdo, Juan; Schmidt, Remo; Mäser, Pascal; Rentsch, Doris; Sigel, Erwin and Bütikofer, Peter

Subjects:

500 Science > 580 Plants (Botany)
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0166-6851

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

12 Feb 2014 08:08

Last Modified:

08 Sep 2015 12:07

Publisher DOI:

10.1016/j.molbiopara.2013.05.007

Uncontrolled Keywords:

Trypanosoma, Choline, Transport, Phosphatidylcholine, Kinetics

BORIS DOI:

10.7892/boris.38669

URI:

https://boris.unibe.ch/id/eprint/38669

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