A versatile proline/alanine transporter in the unicellular pathogen Leishmania donovani regulates amino acid homoeostasis and osmotic stress responses

Inbar, Ehud; Schlisselberg, Doreen; Suter Grotemeyer, Marianne; Rentsch, Doris; Zilberstein, Dan (2013). A versatile proline/alanine transporter in the unicellular pathogen Leishmania donovani regulates amino acid homoeostasis and osmotic stress responses. Biochemical journal, 449(2), pp. 555-566. London: Portland Press 10.1042/BJ20121262

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Unlike all other organisms, parasitic protozoa of the family Trypanosomatidae maintain a large cellular pool of proline that, together with the alanine pool, serve as alternative carbon sources as well as reservoirs of organic osmolytes. These reflect adaptation to their insect vectors whose haemolymphs are exceptionally rich in the two amino acids. In the present study we identify and characterize a new neutral amino acid transporter, LdAAP24, that translocates proline and alanine across the Leishmania donovani plasma membrane. This transporter fulfils multiple functions: it is the sole supplier for the intracellular pool of proline and contributes to the alanine pool; it is essential for cell volume regulation after osmotic stress; and it regulates the transport and homoeostasis of glutamate and arginine, none of which are its substrates. Notably, we provide evidence that proline and alanine exhibit different roles in the parasitic response to hypotonic shock; alanine affects swelling, whereas proline influences the rate of volume recovery. On the basis of our data we suggest that LdAAP24 plays a key role in parasite adaptation to its varying environments in host and vector, a phenomenon essential for successful parasitism.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Inbar, Ehud; Suter, Marianne and Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0264-6021

Publisher:

Portland Press

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

14 Feb 2014 15:42

Last Modified:

10 Jul 2017 16:08

Publisher DOI:

10.1042/BJ20121262

Uncontrolled Keywords:

Amino acid transport, Leishmania, Osmoregulation, Parasitic protozoa

BORIS DOI:

10.7892/boris.38678

URI:

https://boris.unibe.ch/id/eprint/38678

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