The pollen tube: a soft shell with a hard core

Vogler, Hannes; Draeger, Christian; Weber, Alain; Felekis, Dimitris; Eichenberger, Christof; Routier, Anne-Lise; Boisson-Dernier, Aurélien; Ringli, Christoph; Nelson, Bradley J.; Smith, Richard Simon; Grossniklaus, Ueli (2013). The pollen tube: a soft shell with a hard core. The Plant Journal, 73(4), pp. 617-627. Oxford: Blackwell Science 10.1111/tpj.12061

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Plant cell expansion is controlled by a fine-tuned balance between intracellular turgor pressure, cell wall loosening and cell wall biosynthesis. To understand these processes, it is important to gain in-depth knowledge of cell wall mechanics. Pollen tubes are tip-growing cells that provide an ideal system to study mechanical properties at the single cell level. With the available approaches it was not easy to measure important mechanical parameters of pollen tubes, such as the elasticity of the cell wall. We used a cellular force microscope (CFM) to measure the apparent stiffness of lily pollen tubes. In combination with a mechanical model based on the finite element method (FEM), this allowed us to calculate turgor pressure and cell wall elasticity, which we found to be around 0.3 MPa and 20–90 MPa, respectively. Furthermore, and in contrast to previous reports, we showed that the difference in stiffness between the pollen tube tip and the shank can be explained solely by the geometry of the pollen tube. CFM, in combination with an FEM-based model, provides a powerful method to evaluate important mechanical parameters of single, growing cells. Our findings indicate that the cell wall of growing pollen tubes has mechanical properties similar to rubber. This suggests that a fully turgid pollen tube is a relatively stiff, yet flexible cell that can react very quickly to obstacles or attractants by adjusting the direction of growth on its way through the female transmitting tissue.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development

UniBE Contributor:

Weber, Alain; Routier, Anne-Lise and Smith, Richard Simon

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0960-7412

Publisher:

Blackwell Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

07 Dec 2013 00:18

Last Modified:

24 Nov 2015 13:58

Publisher DOI:

10.1111/tpj.12061

PubMed ID:

23106269

Uncontrolled Keywords:

pollen tube; cell wall; turgor pressure; Young's modulus; cellular force microscope; finite element method

BORIS DOI:

10.7892/boris.38692

URI:

https://boris.unibe.ch/id/eprint/38692

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