Mechano-Chemical Aspects of Organ Formation in Arabidopsis thaliana: The Relationship between Auxin and Pectin

Braybrook, Siobhan A.; Peaucelle, Alexis (2013). Mechano-Chemical Aspects of Organ Formation in Arabidopsis thaliana: The Relationship between Auxin and Pectin. PLoS ONE, 8(3), e57813. Lawrence, Kans.: Public Library of Science 10.1371/journal.pone.0057813

[img]
Preview
Text
PLOSone_e57813.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (6MB) | Preview

How instructive signals are translated into robust and predictable changes in growth is a central question in developmental biology. Recently, much interest has centered on the feedback between chemical instructions and mechanical changes for pattern formation in development. In plants, the patterned arrangement of aerial organs, or phyllotaxis, is instructed by the phytohormone auxin; however, it still remains to be seen how auxin is linked, at the apex, to the biochemical and mechanical changes of the cell wall required for organ outgrowth. Here, using Atomic Force Microscopy, we demonstrate that auxin reduces tissue rigidity prior to organ outgrowth in the shoot apex of Arabidopsis thaliana, and that the de-methyl-esterification of pectin is necessary for this reduction. We further show that development of functional organs produced by pectin-mediated ectopic wall softening requires auxin signaling. Lastly, we demonstrate that coordinated localization of the auxin transport protein, PIN1, is disrupted in a naked-apex produced by increasing cell wall rigidity. Our data indicates that a feedback loop between the instructive chemical auxin and cell wall mechanics may play a crucial role in phyllotactic patterning.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Braybrook, Siobhan

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

24 Oct 2013 22:44

Last Modified:

09 Dec 2014 21:14

Publisher DOI:

10.1371/journal.pone.0057813

BORIS DOI:

10.7892/boris.38697

URI:

https://boris.unibe.ch/id/eprint/38697

Actions (login required)

Edit item Edit item
Provide Feedback