Neonatal steroids induce a down-regulation of tenascin-C and elastin and cause a deceleration of the first phase and an acceleration of the second phase of lung alveolarization

Roth-Kleiner, Matthias; Berger, Thomas M; Gremlich, Sandrine; Tschanz, Stefan A.; Mund, Sonja; Post, Martin; Stampanoni, Marco; Schittny, Johannes (2014). Neonatal steroids induce a down-regulation of tenascin-C and elastin and cause a deceleration of the first phase and an acceleration of the second phase of lung alveolarization. Histochemistry and cell biology, 141(1), pp. 75-84. Berlin: Springer 10.1007/s00418-013-1132-7

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Pre- and postnatal corticosteroids are often used in perinatal medicine to improve pulmonary function in preterm infants. To mimic this clinical situation, newborn rats were treated systemically with dexamethasone (Dex), 0.1-0.01 mg/kg/day on days P1-P4. We hypothesized that postnatal Dex may have an impact on alveolarization by interfering with extracellular matrix proteins and cellular differentiation. Morphological alterations were observed on 3D images obtained by high-resolution synchrotron radiation X-ray tomographic microscopy. Alveolarization was quantified stereologically by estimating the formation of new septa between days P4 and P60. The parenchymal expression of tenascin-C (TNC), smooth muscle actin (SMA), and elastin was measured by immunofluorescence and gene expression for TNC by qRT-PCR. After Dex treatment, the first phase of alveolarization was significantly delayed between days P6 and P10, whereas the second phase was accelerated. Elastin and SMA expressions were delayed by Dex treatment, whereas TNC expression was delayed and prolonged. A short course of neonatal steroids impairs the first phase of alveolarization, most likely by altering the TNC and elastin expression. Due to an overshooting catch-up during the second phase of alveolarization, the differences disappear when the animals reach adulthood.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Tschanz, Stefan A.; Mund, Sonja and Schittny, Johannes

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0948-6143

Publisher:

Springer

Language:

English

Submitter:

Johannes Schittny

Date Deposited:

13 Jan 2014 14:59

Last Modified:

04 Jan 2015 00:04

Publisher DOI:

10.1007/s00418-013-1132-7

PubMed ID:

23912843

BORIS DOI:

10.7892/boris.38873

URI:

https://boris.unibe.ch/id/eprint/38873

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