Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry

Kuhnert, Peter; Bisgaard, Magne; Korczak, Bozena M.; Schwendener, Sybille; Christensen, Henrik; Frey, Joachim (2012). Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry. Journal of microbiological methods, 89(1), pp. 1-7. Amsterdam: Elsevier 10.1016/j.mimet.2012.02.001

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Species of the family Pasteurellaceae play an important role as primary or opportunistic animal pathogens. In veterinary diagnostic laboratories identification of this group of bacteria is mainly done by phenotypic assays while genetic identification based on housekeeping genes is mostly used for research and particularly important diagnostic samples. MALDI-TOF MS seems to represent a promising alternative to the currently practiced cumbersome, phenotypic diagnostics carried out in many veterinary diagnostic laboratories. We therefore assessed its application for animal associated members of the family Pasteurellaceae. The Bruker Biotyper 3.0 database was complemented with reference spectra of clinically relevant as well as commensal animal Pasteurellaceae species using generally five strains per species or subspecies and tested for its diagnostic potential with additional, well characterized field isolates. About 250 strains comprising 15 genera and more than 40 species and subspecies were included in the study, covering most representatives of the family. A high discrimination at the genus and species level was observed. Problematic discrimination was only observed with some closely related species and subspecies. MALDI-TOF MS was shown to represent a highly potent method for the diagnosis of this group of animal pathogens, combining speed, precision and low running costs.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter; Korczak, Bozena; Schwendener, Sybille and Frey, Joachim

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0167-7012

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

18 Dec 2013 11:19

Last Modified:

03 Mar 2015 15:34

Publisher DOI:

10.1016/j.mimet.2012.02.001

PubMed ID:

22343217

Uncontrolled Keywords:

MALDI-TOF MS; Diagnosis; Veterinary medicine; Molecular identification; Pasteurellaceae

BORIS DOI:

10.7892/boris.39038

URI:

https://boris.unibe.ch/id/eprint/39038

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