Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish

Burr, Sarah Ellen; Gobeli, Stefanie; Kuhnert, Peter; Goldschmidt-Clermont, Elinor; Frey, Joachim (2010). Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish. International journal of systematic and evolutionary microbiology, 60(Pt 12), pp. 2753-7. Reading, UK: Society for General Microbiology SGM 10.1099/ijs.0.011692-0

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Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835(T) and JF4413, produced non-diffusible green pigment following 4-5 days incubation on Luria-Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C(16 : 1)ω7c and/or C(15 : 0) iso 2-OH), C(16 : 0) and C(18 : 1)ω7c. The DNA G+C content was 62.9 mol%. Sequence analysis of the 16S rRNA gene indicated 100 % sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the Pseudomonas chlororaphis subspecies: P. chlororaphis subsp. chlororaphis (99.84 %), P. chlororaphis subsp. aurantiaca (99.75 %) and P. chlororaphis subsp. aureofaciens (99.40 %). While DNA-DNA relatedness confirmed the placement of strains JF3835(T) and JF4413 as members of the species P. chlororaphis, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835(T) and JF4413 represent a novel subspecies of the species P. chlororaphis, for which the name Pseudomonas chlororaphis subsp. piscium subsp. nov. is proposed. The type strain is JF3835(T) (=NCIMB 14478(T)=DSM 21509(T)).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Burr, Sarah Ellen; Gobeli, Stefanie; Kuhnert, Peter and Frey, Joachim

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

15 Jan 2014 12:16

Last Modified:

03 Mar 2015 15:31

Publisher DOI:

10.1099/ijs.0.011692-0

PubMed ID:

20061493

BORIS DOI:

10.7892/boris.39068

URI:

https://boris.unibe.ch/id/eprint/39068

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