Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals

Dousse, Florence; Thomann, Andreas; Brodard, Isabelle; Korczak, Bozena M.; Schlatter, Yvonne; Kuhnert, Peter; Miserez, Raymond François; Frey, Joachim (2008). Routine phenotypic identification of bacterial species of the family Pasteurellaceae isolated from animals. Journal of veterinary diagnostic investigation, 20(6), pp. 716-724. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063870802000602

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Pasteurellaceae are bacteria with an important role as primary or opportunistic, mainly respiratory, pathogens in domestic and wild animals. Some species of Pasteurellaceae cause severe diseases with high economic losses in commercial animal husbandry and are of great diagnostic concern. Because of new data on the phylogeny of Pasteurellaceae, their taxonomy has recently been revised profoundly, thus requiring an improved phenotypic differentiation procedure to identify the individual species of this family. A new and simplified procedure to identify species of Actinobacillus, Avibacterium, Gallibacterium, Haemophilus, Mannheimia, Nicoletella, and Pasteurella, which are most commonly isolated from clinical samples of diseased animals in veterinary diagnostic laboratories, is presented in the current study. The identification procedure was evaluated with 40 type and reference strains and with 267 strains from routine diagnostic analysis of various animal species, including 28 different bacterial species. Type, reference, and field strains were analyzed by 16S ribosomal RNA (rrs) and rpoB gene sequencing for unambiguous species determination as a basis to evaluate the phenotypic differentiation schema. Primary phenotypic differentiation is based on beta-nicotinamide adenine dinucleotide (beta-NAD) dependence and hemolysis, which are readily determined on the isolation medium. The procedure divides the 28 species into 4 groups for which particular biochemical reactions were chosen to identify the bacterial species. The phenotypic identification procedure allowed researchers to determine the species of 240 out of 267 field strains. The procedure is an easy and cost-effective system for the rapid identification of species of the Pasteurellaceae family isolated from clinical specimens of animals.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Dousse, Florence; Thomann, Andreas; Brodard, Isabelle; Korczak, Bozena; Schlatter, Yvonne; Kuhnert, Peter; Miserez, Raymond François and Frey, Joachim

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1040-6387

Publisher:

American Association of Veterinary Laboratory Diagnosticians AAVLD

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

21 Jan 2014 10:22

Last Modified:

03 Mar 2015 15:26

Publisher DOI:

10.1177/104063870802000602

PubMed ID:

18987220

Uncontrolled Keywords:

Diagnostics, differentiation, Pasteurellaceae, respiratory pathogens, taxonomy

BORIS DOI:

10.7892/boris.39076

URI:

https://boris.unibe.ch/id/eprint/39076

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