Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences

Küpfer, Mara; Kuhnert, Peter; Korczak, Bozena; Peduzzi, Raffaele; Demarta, Antonella (2006). Genetic relationships of Aeromonas strains inferred from 16S rRNA, gyrB and rpoB gene sequences. International journal of systematic and evolutionary microbiology, 56(12), pp. 2743-2751. Reading, UK: Society for General Microbiology SGM 10.1099/ijs.0.63650-0

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Genetic relationships among bacterial strains belonging to the genus Aeromonas were inferred from 16S rRNA, gyrB and rpoB gene sequences. Twenty-eight type or collection strains of the recognized species or subspecies and 33 Aeromonas strains isolated from human and animal specimens as well as from environmental samples were included in the study. As reported previously, the 16S rRNA gene sequence is highly conserved within the genus Aeromonas, having only limited resolution for this very tight group of species. Analysis of a 1.1 kb gyrB sequence confirmed that this gene has high resolving power, with maximal interspecies divergence of 15.2 %. Similar results were obtained by sequencing only 517 bp of the rpoB gene, which showed maximal interspecies divergence of 13 %. The topologies of the gyrB- and rpoB-derived trees were similar. The results confirm the close relationship of species within the genus Aeromonas and show that a phylogenetic approach including several genes is suitable for improving the complicated taxonomy of the genus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter and Korczak, Bozena

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

22 Jan 2014 13:44

Last Modified:

10 Aug 2015 10:52

Publisher DOI:

10.1099/ijs.0.63650-0

PubMed ID:

17158971

Uncontrolled Keywords:

HG, hybridization group

BORIS DOI:

10.7892/boris.39086

URI:

https://boris.unibe.ch/id/eprint/39086

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