[Field validation of a real-time PCR test for the detection of Mycoplasma hyopneumoniae in nasal swabs of live pigs]

Zeeh, Friederike; Kuhnert, Peter; Miserez, Raymond François; Doherr, Marcus; Zimmermann, Werner (2005). [Field validation of a real-time PCR test for the detection of Mycoplasma hyopneumoniae in nasal swabs of live pigs]. Schweizer Archiv für Tierheilkunde, 147(9), pp. 373-379. Bern: Huber 10.1024/0036-7281.147.09.373

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Enzootic pneumonia (EP) of pigs, caused by Mycoplasma hyopneumoniae has been a notifiable disease in Switzerland since May 2003. The diagnosis of EP has been based on multiple methods, including clinical, bacteriological and epidemiological findings as well as pathological examination of lungs (mosaic diagnosis). With the recent development of a real-time PCR (rtPCR) assay with 2 target sequences a new detection method for M. hyopneumoniae became available. This assay was tested for its applicability to nasal swab material from live animals. Pigs from 74 herds (average 10 pigs per herd) were tested. Using the mosaic diagnosis, 22 herds were classified as EP positive and 52 as EP negative. From the 730 collected swab samples we were able to demonstrate that the rtPCR test was 100% specific. In cases of cough the sensitivity on herd level of the rtPCR is 100%. On single animal level and in herds without cough the sensitivity was lower. In such cases, only a positive result would be proof for an infection with M. hyopneumoniae. Our study shows that the rtPCR on nasal swabs from live pigs allows a fast and accurate diagnosis in cases of suspected EP.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Swine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DVK - Clinical Research (discontinued)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Zeeh, Friederike; Kuhnert, Peter; Miserez, Raymond François; Doherr, Marcus and Zimmermann, Werner

Subjects:

600 Technology > 630 Agriculture

ISSN:

0036-7281

Publisher:

Huber

Language:

German

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

21 Jan 2014 10:26

Last Modified:

03 Feb 2015 11:55

Publisher DOI:

10.1024/0036-7281.147.09.373

PubMed ID:

16180340

Uncontrolled Keywords:

Mycoplasma hyopneumoniae, Diagnose, real-time PCR, Nasentupfer, lebende Schweine

BORIS DOI:

10.7892/boris.39087

URI:

https://boris.unibe.ch/id/eprint/39087

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