Phylogeny of the family Pasteurellaceae based on rpoB sequences

Korczak, Bozena; Christensen, Henrik; Emler, Stefan; Frey, Joachim; Kuhnert, Peter (2004). Phylogeny of the family Pasteurellaceae based on rpoB sequences. International journal of systematic and evolutionary microbiology, 54(4), pp. 1393-1399. Reading, UK: Society for General Microbiology SGM 10.1099/ijs.0.03043-0

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Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Korczak, Bozena; Frey, Joachim and Kuhnert, Peter

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

30 Jan 2014 11:56

Last Modified:

10 Aug 2015 10:45

Publisher DOI:

10.1099/ijs.0.03043-0

PubMed ID:

15280320

BORIS DOI:

10.7892/boris.39117

URI:

https://boris.unibe.ch/id/eprint/39117

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