Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering

Kuhnert, Peter; Frey, Joachim; Lang, Niklaus P.; Mayfield, Lisa (2002). Phylogenetic analysis of Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis clinical strains reveals a clear species clustering. International journal of systematic and evolutionary microbiology, 52(4), pp. 1391-1395. Reading, UK: Society for General Microbiology SGM 10.1099/ijs.0.02021-0

[img] Text
Prevotella.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (238kB) | Request a copy

Prevotella nigrescens, Prevotella intermedia and Porphyromonas gingivalis are oral pathogens from the family Bacteroidaceae, regularly isolated from cases of gingivitis and periodontitis. In this study, the phylogenetic variability of these three bacterial species was investigated by means of 16S rRNA (rrs) gene sequence comparisons of a set of epidemiologically and geographically diverse isolates. For each of the three species, the rrs gene sequences of 11 clinical isolates as well as the corresponding type strains was determined. Comparison of all rrs sequences obtained with those of closely related species revealed a clear clustering of species, with only a little intraspecies variability but a clear difference in the rrs gene with respect to the next related taxon. The results indicate that the three species form stable, homogeneous genetic groups, which favours an rrs-based species identification of these oral pathogens. This is especially useful given the 7% sequence divergence between Prevotella intermedia and Prevotella nigrescens, since phenotypic distinction between the two Prevotella species is inconsistent or involves techniques not applicable in routine identification.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter; Frey, Joachim and Lang, Niklaus Peter

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1466-5026

Publisher:

Society for General Microbiology SGM

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

30 Jan 2014 10:50

Last Modified:

10 Aug 2015 10:34

Publisher DOI:

10.1099/ijs.0.02021-0

PubMed ID:

12148655

Uncontrolled Keywords:

phylogeny, variation, 16S rRNA, oral pathogens

BORIS DOI:

10.7892/boris.39130

URI:

https://boris.unibe.ch/id/eprint/39130

Actions (login required)

Edit item Edit item
Provide Feedback