Analysis of non-porcine isolates of Actinobacillus suis

Jeannotte, Marie-Eve; Slavić, Durda; Frey, Joachim; Kuhnert, Peter; MacInnes, Janet I (2002). Analysis of non-porcine isolates of Actinobacillus suis. Veterinary microbiology, 85(1), pp. 83-93. Amsterdam: Elsevier 10.1016/S0378-1135(01)00484-9

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Twenty-four Actinobacillus suis isolates obtained from several species of non-porcine mammals were compared to the representative porcine strains, ATCC 15557 (serotype O1) and H89-1173 (serotype O2), by O serotyping, DNA fingerprinting, PCR amplification of apxICA, apxIICA and apxIIICA toxin genes and by rrs (16S rRNA) gene sequencing. Only two strains, both equine, reacted with O1 antiserum while two others, one canine and the other feline, reacted with O2 antiserum. One equine strain reacted weakly with both antisera. No amplification of apx genes was found with the non-porcine O1 or the "not O1/O2" strains but amplification of the apxICA and apxIICA genes was observed with the two O2 strains. In addition, these two O2 strains had both BamHI and BglII fingerprints that were very similar to the porcine O2 reference strain, H89-1173 and rrs gene sequences that were identical to the A. suis reference strain ATCC 15557. Taken together, these data suggest that although many non-porcine A. suis isolates are not A. suis (sensu stricto), some isolates are genotypically as well as phenotypically similar to A. suis.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Frey, Joachim and Kuhnert, Peter

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

24 Jan 2014 08:52

Last Modified:

12 Jan 2015 03:42

Publisher DOI:

10.1016/S0378-1135(01)00484-9

PubMed ID:

11792495

Uncontrolled Keywords:

Actinobacillus suis, Serotyping, DNA fingerprinting, rrs, apx

BORIS DOI:

10.7892/boris.39133

URI:

https://boris.unibe.ch/id/eprint/39133

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