Phylogenetic analysis of Pasteurella multocida subspecies and molecular identification of feline P. multocida subsp. septica by 16S rRNA gene sequencing

Kuhnert, Peter; Boerlin, P.; Emler, S.; Krawinkler, M.; Frey, Joachim (2000). Phylogenetic analysis of Pasteurella multocida subspecies and molecular identification of feline P. multocida subsp. septica by 16S rRNA gene sequencing. International journal of medical microbiology IJMM, 290(7), pp. 599-604. Elsevier 10.1016/S1438-4221(00)80008-9

[img] Text
Pseptica_phylo.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (195kB) | Request a copy

Pasteurella multocida is commonly found in the oral cavity of cats and dogs. In humans it is known as an opportunistic pathogen after bites from these animals. Phenotypic identification of P. multocida based on biochemical reactions is often limited and usually only done on a species level, even though 3 subspecies are described. For molecular taxonomy and diagnostic purposes a phylogenetic analysis of the three subspecies of P. multocida based on their 16S rRNA (rrs) gene sequence was therefore carried out. We found P. multocida subsp. septica on a distinguished branch on the phylogenetic tree of Pasteurellaceae, due to a 1.5% divergence of its rrs gene compared to the two other, more closely related subspecies multocida and gallicida. This phylogenetic divergence can be used for the identification of P. multocida subsp. septica by rrs gene determination since they form a phylogenetically well isolated and defined group as shown with a set of feline isolates. Comparison to routine phenotypic identification shows the advantage of the sequence-based identification over conventional methods. It is therefore helpful for future unambiguous identification and molecular taxonomy of P. multocida as well as for epidemiological investigations.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kuhnert, Peter and Frey, Joachim

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1438-4221

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

05 Jun 2014 16:04

Last Modified:

10 Aug 2015 10:06

Publisher DOI:

10.1016/S1438-4221(00)80008-9

PubMed ID:

11200541

BORIS DOI:

10.7892/boris.39155

URI:

https://boris.unibe.ch/id/eprint/39155

Actions (login required)

Edit item Edit item
Provide Feedback