Detection of the ADP-ribosyltransferase toxin gene (cdtA) and its activity in Clostridium difficile isolates from Equidae

Braun, Martin; Herholz, Cornelia; Straub, Reto; Choisat, Béatrice; Frey, Joachim; Nicolet, Jacques; Kuhnert, Peter (2000). Detection of the ADP-ribosyltransferase toxin gene (cdtA) and its activity in Clostridium difficile isolates from Equidae. FEMS microbiology letters, 184(1), pp. 29-33. Malden, Mass.: Blackwell Publishing Ltd 10.1111/j.1574-6968.2000.tb08985.x

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Clostridium difficile is an antibiotic-associated emerging pathogen of humans and animals. Thus far three toxins of C. difficile have been described: an enterotoxin (ToxA), a cytotoxin (ToxB) and an ADP-ribosyltransferase (CDT). In the present work we describe the first isolation of CDT producing C. difficile from Equidae with gastro-intestinal disease. Out of 17 C. difficile strains isolated from Equidae, 11 were positive for the genes tcdA and tcdB encoding ToxA and ToxB. In addition four of these 11 isolates were positive for the cdtA gene encoding the catalytic subunit of the ADP-ribosyltransferase CDT. Interestingly none of the isolates derived from canines (41 isolates) and felines (4 isolates) harboured the cdtA gene. In C. difficile field isolates which contained the cdtA gene, ADP-ribosyltransferase activity could also be detected in culture supernatants indicating expression and secretion of CDT. All strains were associated with intestinal disorders, but no association was found for the occurrence of toxins with a specific clinical diagnosis.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic

UniBE Contributor:

Straub, Reto; Frey, Joachim and Kuhnert, Peter

Subjects:

500 Science
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0378-1097

Publisher:

Blackwell Publishing Ltd

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

15 Jan 2014 11:09

Last Modified:

27 Apr 2018 09:11

Publisher DOI:

10.1111/j.1574-6968.2000.tb08985.x

PubMed ID:

10689161

Uncontrolled Keywords:

Clostridium difficile; ADP-ribosyltransferase toxin; Diseased Equidae

BORIS DOI:

10.7892/boris.39160

URI:

https://boris.unibe.ch/id/eprint/39160

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