Optimized Demineralization Technique for the Measurement of Stable Isotope Ratios of Nonexchangeable H in Soil Organic Matter

Ruppenthal, Marc; Oelmann, Yvonne; Wilcke, Wolfgang (2013). Optimized Demineralization Technique for the Measurement of Stable Isotope Ratios of Nonexchangeable H in Soil Organic Matter. Environmental science & technology, 47(2), pp. 949-957. ACS Publications 10.1021/es303448g

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To make use of the isotope ratio of nonexchangeable hydrogen (δ2Hn (nonexchangeable)) of bulk soil organic matter (SOM), the mineral matrix (containing structural water of clay minerals) must be separated from SOM and samples need to be analyzed after H isotope equilibration. We present a novel technique for demineralization of soil samples with HF and dilute HCl and recovery of the SOM fraction solubilized in the HF demineralization solution via solid-phase extraction. Compared with existing techniques, organic C (Corg) and organic N (Norg) recovery of demineralized SOM concentrates was significantly increased (Corg recovery using existing techniques vs new demineralization method: 58% vs 78%; Norg recovery: 60% vs 78%). Chemicals used for the demineralization treatment did not affect δ2Hn values as revealed by spiking with deuterated water. The new demineralization method minimized organic matter losses and thus artificial H isotope fractionation, opening up the opportunity to use δ2Hn analyses of SOM as a new tool in paleoclimatology or geospatial forensics.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Institute of Geography

UniBE Contributor:

Wilcke, Wolfgang

Subjects:

900 History > 910 Geography & travel

ISSN:

0013-936X

Publisher:

ACS Publications

Language:

English

Submitter:

Monika Wälti-Stampfli

Date Deposited:

17 Jan 2014 09:16

Last Modified:

19 Oct 2015 09:54

Publisher DOI:

10.1021/es303448g

BORIS DOI:

10.7892/boris.39660

URI:

https://boris.unibe.ch/id/eprint/39660

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