Design-based stereology: Planning, volumetry and sampling are crucial steps for a successful study

Tschanz, Stefan A.; Schneider, Jan Philipp; Kundson, Lars (2014). Design-based stereology: Planning, volumetry and sampling are crucial steps for a successful study. Annals of anatomy - Anatomischer Anzeiger, 196(1), pp. 3-11. Elsevier 10.1016/j.aanat.2013.04.011

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Quantitative data obtained by means of design-based stereology can add valuable information to studies performed on a diversity of organs, in particular when correlated to functional/physiological and biochemical data. Design-based stereology is based on a sound statistical background and can be used to generate accurate data which are in line with principles of good laboratory practice. In addition, by adjusting the study design an appropriate precision can be achieved to find relevant differences between groups. For the success of the stereological assessment detailed planning is necessary. In this review we focus on common pitfalls encountered during stereological assessment. An exemplary workflow is included, and based on authentic examples, we illustrate a number of sampling principles which can be implemented to obtain properly sampled tissue blocks for various purposes.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Anatomy

UniBE Contributor:

Tschanz, Stefan A.

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0940-9602

Publisher:

Elsevier

Language:

English

Submitter:

Beatrice Itten

Date Deposited:

13 Jan 2014 12:21

Last Modified:

13 Dec 2014 03:54

Publisher DOI:

10.1016/j.aanat.2013.04.011

PubMed ID:

23769130

Uncontrolled Keywords:

Cavalieri method, Design-based stereology, Fractionator, Systematic uniform random sample, Volumetry

BORIS DOI:

10.7892/boris.39814

URI:

https://boris.unibe.ch/id/eprint/39814

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