In vitro evaluation of demineralized freeze-dried bone allograft in combination with enamel matrix derivative

Miron, Richard; Bosshardt, Dieter; Laugisch, Oliver; Dard, Michel; Gemperli, Anja C; Buser, Daniel; Gruber, Reinhard; Sculean, Anton (2013). In vitro evaluation of demineralized freeze-dried bone allograft in combination with enamel matrix derivative. Journal of periodontology, 84(11), pp. 1646-54. American Academy of Periodontology 10.1902/jop.2013.120574

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BACKGROUND Preclinical and clinical studies suggest that a combination of enamel matrix derivative (EMD) with demineralized freeze-dried bone allograft (DFDBA) may improve periodontal wound healing and regeneration. To date, no single study has characterized the effects of this combination on in vitro cell behavior. The aim of this study is to test the ability of EMD to adsorb to the surface of DFDBA particles and determine the effect of EMD coating on downstream cellular pathways such as adhesion, proliferation, and differentiation of primary human osteoblasts and periodontal ligament (PDL) cells. METHODS DFDBA particles were precoated with EMD or human blood and analyzed for protein adsorption patterns via scanning electron microscopy. Cell attachment and proliferation were quantified using a commercial assay. Cell differentiation was analyzed using real-time polymerase chain reaction for genes encoding Runx2, alkaline phosphatase, osteocalcin, and collagen 1α1, and mineralization was assessed using alizarinred staining. RESULTS Analysis of cell attachment revealed no significant differences among control, blood-coated, and EMD-coated DFDBA particles. EMD significantly increased cell proliferation at 3 and 5 days after seeding for both osteoblasts and PDL cells compared to control and blood-coated samples. Moreover, there were significantly higher messenger ribonucleic acid levels of osteogenic differentiation markers, including collagen 1α1, alkaline phosphatase, and osteocalcin, in osteoblasts and PDL cells cultured on EMD-coated DFDBA particles at 3, 7, and 14 days. CONCLUSION The results suggest that the addition of EMD to DFDBA particles may influence periodontal regeneration by stimulating PDL cell and osteoblast proliferation and differentiation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
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UniBE Contributor:

Miron, Richard; Bosshardt, Dieter; Laugisch, Oliver; Buser, Daniel; Gruber, Reinhard and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-3492

Publisher:

American Academy of Periodontology

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

17 Feb 2014 12:05

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1902/jop.2013.120574

PubMed ID:

23347347

URI:

https://boris.unibe.ch/id/eprint/40396

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