Assessment of metabolic stability using the rainbow trout (Oncorhynchus mykiss) liver S9 fraction

Johanning, K.; Hancock, G.; Escher, B.; Adekola, A.; Bernhard, M. J.; Cowan-Ellsberry, C.; Domoradzki, J.; Dyer, S.; Eickhoff, C.; Embry, M.; Erhardt, S.; Fitzsimmons, P.; Halder, M.; Hill, J.; Holden, D.; Johnson, R.; Rutishauser, S.; Segner, Helmut; Schultz, I. and Nichols, J. (2012). Assessment of metabolic stability using the rainbow trout (Oncorhynchus mykiss) liver S9 fraction. In: Current Protocols in Toxicology Chapter 14 (Unit 14.10.1-28). Wiley 10.1002/0471140856.tx1410s53

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Standard protocols are given for assessing metabolic stability in rainbow trout using the liver S9 fraction. These protocols describe the isolation of S9 fractions from trout livers, evaluation of metabolic stability using a substrate depletion approach, and expression of the result as in vivo intrinsic clearance. Additional guidance is provided on the care and handling of test animals, design and interpretation of preliminary studies, and development of analytical methods. Although initially developed to predict metabolism impacts on chemical accumulation by fish, these procedures can be used to support a broad range of scientific and risk assessment activities including evaluation of emerging chemical contaminants and improved interpretation of toxicity testing results. These protocols have been designed for rainbow trout and can be adapted to other species as long as species-specific considerations are modified accordingly (e.g., fish maintenance and incubation mixture temperature). Rainbow trout is a cold-water species. Protocols for other species (e.g., carp, a warm-water species) can be developed based on these procedures as long as the specific considerations are taken into account.

Item Type:

Book Section (Book Chapter)


05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Segner, Helmut


600 Technology > 630 Agriculture








Pascal Escher

Date Deposited:

31 Jan 2014 09:27

Last Modified:

31 Jul 2017 08:31

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Animals, Aquaculture, Biological Assay/methods, *Metabolic Detoxication, Phase I, *Metabolic Detoxication, Phase II, Microsomes, Liver/*drug effects/metabolism, Models, Animal, *Oncorhynchus mykiss, Specimen Handling/methods, Toxicity Tests/*methods, Xenobiotics/*toxicity




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