Estrogen Modulates Hepatic Gene Expression and Survival of Rainbow Trout Infected with Pathogenic Bacteria Yersinia ruckeri

Wenger, Michael; Krasnov, A.; Skugor, S.; Goldschmidt-Clermont, E.; Sattler, Ursula; Afanasyev, S.; Segner, Helmut (2012). Estrogen Modulates Hepatic Gene Expression and Survival of Rainbow Trout Infected with Pathogenic Bacteria Yersinia ruckeri. Marine Biotechnoloy, 14(5), pp. 530-543. Springer 10.1007/s10126-012-9473-0

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In the aquatic environment, fish are exposed to various stimuli at once and have developed different response mechanisms to deal with these multiple stimuli. The current study assessed the combined impacts of estrogens and bacterial infection on the physiological status of fish. Juvenile rainbow trout were exposed to two different concentrations of 17 beta-estradiol (E2) (2 or 20 mg/kg feed) and then infected with three concentrations of Yersinia ruckeri, a bacterial pathogen causing massive losses in wild and farmed salmonid populations. Organism-level endpoints to assess the impact of the single and combined treatments included hepatic vitellogenin transcript expression to evaluate the E2 exposure efficiency and survival rate of pathogen-challenged fish. The two E2 doses increased vitellogenin levels within the physiological range. Infection with Y. ruckeri caused mortality of trout, and this effect was significantly enhanced by a simultaneous exposure to high E2 dose. The hormone reduced survival at intermediate and high (10(4) and 10(6) colony forming units, cfu) bacterial concentrations, but not for a low one (10(2) cfu). Analysis of hepatic gene expression profiles by a salmonid 2 k cDNA microarray chip revealed complex regulations of pathways involved in immune responses, stress responses, and detoxicification pathways. E2 markedly reduced the expression of several genes implicated in xenobiotic metabolism. The results suggest that the interaction between pathogen and E2 interfered with the fish's capability of clearing toxic compounds. The findings of the current study add to our understanding of multiple exposure responses in fish.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Wenger, Michael; Sattler, Ursula and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

1436-2228

Publisher:

Springer

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

27 Jan 2014 11:07

Last Modified:

08 Apr 2015 13:09

Publisher DOI:

10.1007/s10126-012-9473-0

PubMed ID:

307710600003

Uncontrolled Keywords:

disease resistance, 17 beta-estradiol, immunity, xenobiotics metabolism, rainbow trout, yersinia ruckeri, breast-cancer, oncorhynchus-mykiss, cytochromes p450, immune-response, 17-beta-estradiol, fish, endocrine, reveals, enzymes, protein

BORIS DOI:

10.7892/boris.40453

URI:

https://boris.unibe.ch/id/eprint/40453

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