Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47

Ye, Roy R.; Lei, Elva N. Y.; Lam, Michael H. W.; Chan, Alice K. Y.; Bo, Jun; van de Merwe, Jason P.; Fong, Amy C. C.; Yang, Michael M. S.; Lee, J. S.; Segner, Helmut E.; Wong, Chris K. C.; Wu, Rudolf S. S.; Au, Doris W. T. (2012). Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47. Environmental science and pollution research, 19(7), pp. 2477-2487. Springer 10.1007/s11356-012-0887-z

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BDE-47 is one of the most widely found congeners of PBDEs in marine environments. The potential immunomodulatory effects of BDE-47 on fish complement system were studied using the marine medaka Oryzias melastigma as a model fish. Three-month-old O. melastigma were subjected to short-term (5 days) and long-term (21 days) exposure to two concentrations of BDE-47 (low dose at 290 +/- 172 ng/day; high dose at 580 +/- 344 ng/day) via dietary uptake of BDE-47 encapsulated in Artemia nauplii. Body burdens of BDE-47 and other metabolic products were analyzed in the exposed and control fish. Only a small amount of debrominated product, BDE-28, was detected, while other metabolic products were all under detection limit. Transcriptional expression of six major complement system genes involved in complement activation: C1r/s (classical pathway), MBL-2 (lectin pathway), CFP (alternative pathway), F2 (coagulation pathway), C3 (the central component of complement system), and C9 (cell lysis) were quantified in the liver of marine medaka. Endogenous expression of all six complement system genes was found to be higher in males than in females (p < 0.05). Upon dietary exposure of marine medaka to BDE-47, expression of all six complement genes were downregulated in males at day 5 (or longer), whereas in females, MBl-2, CFP, and F2 mRNAs expression were upregulated, but C3 and C9 remained stable with exposure time and dose. A significant negative relationship was found between BDE-47 body burden and mRNA expression of C1r/s, CFP, and C3 in male fish (r = -0.8576 to -0.9447). The above findings on changes in complement gene expression patterns indicate the complement system may be compromised in male O. melastigma upon dietary exposure to BDE-47. Distinct gender difference in expression of six major complement system genes was evident in marine medaka under resting condition and dietary BDE-47 challenge. The immunomodulatory effects of BDE-47 on transcriptional expression of these complement components in marine medaka were likely induced by the parent compound instead of biotransformed products. Our results clearly demonstrate that future direction for fish immunotoxicology and risk assessment of immunosuppressive chemicals must include parallel evaluation for both genders.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology

UniBE Contributor:

Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

0944-1344

Publisher:

Springer

Language:

English

Submitter:

Andrea Stettler

Date Deposited:

29 Apr 2014 15:45

Last Modified:

10 Oct 2017 21:33

Publisher DOI:

10.1007/s11356-012-0887-z

Web of Science ID:

306790200003

Uncontrolled Keywords:

pbdes, bde-47, marine medaka, immunomodulatory effects complement system, c3, gender difference polybrominated diphenyl ethers endocrine-disrupting potency brominated flame retardants activation pathway coastal waters component c3 fish model north-sea pbdes responses

BORIS DOI:

10.7892/boris.40456

URI:

https://boris.unibe.ch/id/eprint/40456

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