Antibacterial activity of moxifloxacin on bacteria associated with periodontitis within a biofilm

Tsaousoglou, Phoebus; Nietzsche, Sandor; Cachovan, Georg; Sculean, Anton; Eick, Sigrun (2014). Antibacterial activity of moxifloxacin on bacteria associated with periodontitis within a biofilm. Journal of medical microbiology, 63(2), pp. 284-292. SGM 10.1099/jmm.0.065441-0

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The activity of moxifloxacin was compared with ofloxacin and doxycycline against bacteria associated with periodontitis within a biofilm (single strain and mixed population) in vitro. Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of moxifloxacin, ofloxacin and doxycyline were determined against single strains and mixed populations in a planktonic state. Single-species biofilms of two Porphyromonas gingivalis and two Aggregatibacter actinomycetemcomitans strains and a multi-species biofilm consisting of 12 species were formed for 3 days. The minimal biofilm eradication concentrations (MBECs) were determined after exposing the biofilms to the antibacterials (0.002 - 512 µg ml-1) for 18 h, addition of nutrient broth for 3 days and subsequent subcultivation. Photographs were taken by using confocal laser scanning microscopy and scanning electron microscopy. The MICs and MBCs did not differ between ofloxacin and moxifloxacin against A. actinomycetemcomitans, moxifloxacin was more active than the other tested antibacterials against anaerobes and the mixed population. The single-species biofilms were eradicated by moderate concentrations of the antibacterials, the lowest MBECs were always found for moxifloxacin (2-8 µg ml-1). MBECs against the multi-species biofilms were 128 µg ml-1, >512 µg ml-1 and >512 µg ml-1 for moxifloxacin, ofloxacin and doxycycline, respectively. In summary, moxifloxacin in a topical formulation may have potential as an adjunct to mechanical removal of the biofilms.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Sculean, Anton and Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-2615

Publisher:

SGM

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

30 Apr 2014 15:27

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1099/jmm.0.065441-0

PubMed ID:

24217128

Uncontrolled Keywords:

Anaerobes, Antimicrobials, Biofilms, Oral bacteria

URI:

https://boris.unibe.ch/id/eprint/40644

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