Comparative antibacterial efficacies of hydrodynamic and ultrasonic irrigation systems in vitro

Cachovan, Georg; Schiffner, Ulrich; Altenhof, Saskia; Guentsch, Arndt; Pfister, Wolfgang; Eick, Sigrun (2013). Comparative antibacterial efficacies of hydrodynamic and ultrasonic irrigation systems in vitro. Journal of endodontics, 39(9), pp. 1171-5. Elsevier 10.1016/j.joen.2013.06.008

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INTRODUCTION To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. The in vitro bactericidal effects of a hydrodynamic system and a passive ultrasonic irrigation system were compared. METHODS Single-rooted extracted teeth (n = 250) were contaminated with suspensions of Enterococcus faecalis ATCC 29212, mixed aerobic cultures, or mixed anaerobic cultures. First, the antibacterial effects of the hydrodynamic system (RinsEndo), a passive ultrasonic irrigation system (Piezo smart), and manual rinsing with 0.9% NaCl (the control) were compared. Colony-forming units were counted. Second, the 2 systems were used with 1.5% sodium hypochlorite (NaOCl) alone or NaOCl + 0.2% chlorhexidine (CHX). The colony-forming units in the treated and untreated roots were determined during a period of 5 days. RESULTS Both irrigation systems reduced bacterial numbers more effectively than manual rinsing (P < .001). With NaCl, ultrasonic activated irrigation reduced bacterial counts significantly better than hydrodynamic irrigation (P = .042). The NaOCl + CHX combination was more effective than NaOCl alone for both systems (P < .001), but hydrodynamic irrigation was more effective with NaOCl + CHX than the passive ultrasonic irrigation system. CONCLUSIONS Both irrigation systems, when combined with NaOCl + CHX, removed bacteria from root canals.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Eick, Sigrun


600 Technology > 610 Medicine & health








Eveline Carmen Schuler

Date Deposited:

25 Feb 2014 08:44

Last Modified:

25 Jan 2017 12:15

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Antimicrobial activity, Enterococcus faecalis hydrodynamic irrigation passive ultrasonic irrigation


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