Source attribution of human Campylobacter isolates by MLST and fla-typing and association of genotypes with quinolone resistance

Kittl, Sonja; Heckel, Gerald; Korczak, Bozena; Kuhnert, Peter (2013). Source attribution of human Campylobacter isolates by MLST and fla-typing and association of genotypes with quinolone resistance. PLoS ONE, 8(11), e81796. Public Library of Science 10.1371/journal.pone.0081796

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Campylobacteriosis is the most frequent zoonosis in developed countries and various domestic animals can function as reservoir for the main pathogens Campylobacter jejuni and Campylobacter coli. In the present study we compared population structures of 730 C. jejuni and C. coli from human cases, 610 chicken, 159 dog, 360 pig and 23 cattle isolates collected between 2001 and 2012 in Switzerland. All isolates had been typed with multi locus sequence typing (MLST) and flaB-typing and their genotypic resistance to quinolones was determined. We used complementary approaches by testing for differences between isolates from different hosts with the proportion similarity as well as the fixation index and by attributing the source of the human isolates with Bayesian assignment using the software STRUCTURE. Analyses were done with MLST and flaB data in parallel and both typing methods were tested for associations of genotypes with quinolone resistance. Results obtained with MLST and flaB data corresponded remarkably well, both indicating chickens as the main source for human infection for both Campylobacter species. Based on MLST, 70.9% of the human cases were attributed to chickens, 19.3% to cattle, 8.6% to dogs and 1.2% to pigs. Furthermore we found a host independent association between sequence type (ST) and quinolone resistance. The most notable were ST-45, all isolates of which were susceptible, while for ST-464 all were resistant.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Population Genetics
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Kittl, Sonja; Heckel, Gerald; Korczak, Bozena and Kuhnert, Peter

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

05 Feb 2014 17:14

Last Modified:

23 Apr 2015 16:20

Publisher DOI:

10.1371/journal.pone.0081796

PubMed ID:

24244747

BORIS DOI:

10.7892/boris.40675

URI:

https://boris.unibe.ch/id/eprint/40675

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