Assay of β-hematin formation by malaria parasite

Pandey, A. V.; Singh, N.; Tekwani, B. L.; Puri, S. K.; Chauhan, V. S. (1999). Assay of β-hematin formation by malaria parasite. Journal of pharmaceutical and biomedical analysis, 20(1-2), pp. 203-207. Pergamon Press 10.1016/S0731-7085(99)00021-7

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Novel leads are urgently required for designing antimalarials due to the reduced efficacy of presently available drugs. The malaria parasite has a unique reaction of heme polymerization, which has attracted much attention in the recent past as a target for the design of antimalarial drugs. The process is hampered by non-availability of a proper assay method. Currently available methods are cumbersome and require advanced instrumentation or radioactive substrates. Here, we are describing an assay for hemozoin formation that is simple and reproducible. This assay has routinely been used by us for the identification of potential compounds with antimalarial activity.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital (discontinued) > Forschungsgruppe Endokrinologie/Diabetologie/Metabolik (Pädiatrie) (discontinued)

UniBE Contributor:

Pandey, Amit Vikram

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0731-7085

Publisher:

Pergamon Press

Language:

English

Submitter:

Amit Vikram Pandey

Date Deposited:

25 Aug 2014 12:08

Last Modified:

28 Dec 2014 17:24

Publisher DOI:

10.1016/S0731-7085(99)00021-7

PubMed ID:

10704024

Uncontrolled Keywords:

Hemozoin, Malaria, Plasmodium, Hemoglobin, Heme, Chloroquine

BORIS DOI:

10.7892/boris.41751

URI:

https://boris.unibe.ch/id/eprint/41751

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