Depolymerization of malarial hemozoin: a novel reaction initiated by blood schizontocidal antimalarials

Pandey, Amit V.; Tekwani, Babu L. (1997). Depolymerization of malarial hemozoin: a novel reaction initiated by blood schizontocidal antimalarials. FEBS letters, 402(2-3), pp. 236-240. Elsevier 10.1016/S0014-5793(96)01536-0

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Malaria parasite digests hemoglobin and utilizes the globin part for its nutritional requirements. Heme released as a byproduct of hemoglobin degradation is detoxified by polymerization into a crystalline, insoluble pigment, known as hemozoin. We have identified a novel reaction of depolymerization of hemozoin to heme. This reaction is initiated by the interaction of blood schizonticidal antimalarial drugs with the malarial hemozoin. The reaction has been confirmed, with the purified hemozoin as well as the lysate of the malaria parasite. Pigment breakdown was studied by infrared spectroscopy, thin-layer chromatography and spectrophotometric analysis. It was complete within 2 h of drug exposure, which explains the selective sensitivity of late stages (trophozoites and schizonts) of malarial parasites loaded with the hemozoin pigment to the toxic action of these drugs. It is suggested that the failure of the parasite heme detoxification system due to this reaction results in the accumulation of toxic heme, which alone, or complexed with the antimalarial leads to the death of malaria parasite.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital (discontinued) > Forschungsgruppe Endokrinologie/Diabetologie/Metabolik (Pädiatrie) (discontinued)

UniBE Contributor:

Pandey, Amit Vikram

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0014-5793

Publisher:

Elsevier

Language:

English

Submitter:

Amit Vikram Pandey

Date Deposited:

25 Aug 2014 10:31

Last Modified:

19 Dec 2014 17:16

Publisher DOI:

10.1016/S0014-5793(96)01536-0

PubMed ID:

9037202

Uncontrolled Keywords:

Malaria, Hemozoin, Heme, Antimalarial, Chloroquine

BORIS DOI:

10.7892/boris.41755

URI:

https://boris.unibe.ch/id/eprint/41755

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