Pandey, Amit Vikram; Tekwani, Babu L. (1996). Formation of haemozoin/β-haematin under physiological conditions is not spontaneous. FEBS letters, 393(2-3), pp. 189-192. Elsevier 10.1016/0014-5793(96)00881-2
|
Text
1-s2.0-0014579396008812-main.pdf - Published Version Available under License Publisher holds Copyright. Under an Elsevier user license - Open Archive Download (346kB) | Preview |
Malaria parasite detoxifies free haem, released as a result of haemoglobin digestion, by converting it into an stable, crystalline, black brown pigment known as 'malaria pigment' or 'haemozoin'. Earlier studies have demonstrated the involvement of a parasite-specific enzyme 'haem polymerase' in the formation of haemozoin. However, recently it has been proposed that the polymerization of haem may be a spontaneous process that could take place by incubation of haematin with carboxylic acids (pH 4.2-5.0) even without presence of any parasitic or biological component (FEBS Letters, 352, 54-57 (1994). Here we report that no spontaneous haem polymerization occurs at physiological conditions and the product described in the study mentioned above is not haemozoin/beta-haematin (haem polymer) as characterized by us on the basis of solubility characteristics and thin layer chromatography. The infra-red spectroscopic analysis of the product formed though exhibits the bands corresponding to formation of iron-carboxylate bond, similar to that in haemozoin/beta-haematin, but was identified as haem-acid adduct. Thus polymerization of haem may not occur spontaneously under the reaction conditions corresponding to food vacuoles of the malarial parasite, the physiological site of haemozoin formation.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine > Endocrinology/Metabolic Disorders |
UniBE Contributor: |
Pandey, Amit Vikram |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
0014-5793 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Amit Vikram Pandey |
Date Deposited: |
25 Aug 2014 09:38 |
Last Modified: |
06 Jan 2023 19:33 |
Publisher DOI: |
10.1016/0014-5793(96)00881-2 |
PubMed ID: |
8814287 |
Uncontrolled Keywords: |
Malaria, Haem, Haemozoin, β-Haematin |
BORIS DOI: |
10.7892/boris.41756 |
URI: |
https://boris.unibe.ch/id/eprint/41756 |
Actions (login required)
 |
Edit item |