Formation of haemozoin/β-haematin under physiological conditions is not spontaneous

Pandey, Amit V.; Tekwani, Babu L. (1996). Formation of haemozoin/β-haematin under physiological conditions is not spontaneous. FEBS letters, 393(2-3), pp. 189-192. Elsevier 10.1016/0014-5793(96)00881-2

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Malaria parasite detoxifies free haem, released as a result of haemoglobin digestion, by converting it into an stable, crystalline, black brown pigment known as 'malaria pigment' or 'haemozoin'. Earlier studies have demonstrated the involvement of a parasite-specific enzyme 'haem polymerase' in the formation of haemozoin. However, recently it has been proposed that the polymerization of haem may be a spontaneous process that could take place by incubation of haematin with carboxylic acids (pH 4.2-5.0) even without presence of any parasitic or biological component (FEBS Letters, 352, 54-57 (1994). Here we report that no spontaneous haem polymerization occurs at physiological conditions and the product described in the study mentioned above is not haemozoin/beta-haematin (haem polymer) as characterized by us on the basis of solubility characteristics and thin layer chromatography. The infra-red spectroscopic analysis of the product formed though exhibits the bands corresponding to formation of iron-carboxylate bond, similar to that in haemozoin/beta-haematin, but was identified as haem-acid adduct. Thus polymerization of haem may not occur spontaneously under the reaction conditions corresponding to food vacuoles of the malarial parasite, the physiological site of haemozoin formation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital (discontinued)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Unit Tiefenau Hospital (discontinued) > Forschungsgruppe Endokrinologie/Diabetologie/Metabolik (Pädiatrie) (discontinued)

UniBE Contributor:

Pandey, Amit Vikram

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0014-5793

Publisher:

Elsevier

Language:

English

Submitter:

Amit Vikram Pandey

Date Deposited:

25 Aug 2014 09:38

Last Modified:

22 Dec 2014 20:21

Publisher DOI:

10.1016/0014-5793(96)00881-2

PubMed ID:

8814287

Uncontrolled Keywords:

Malaria, Haem, Haemozoin, β-Haematin

BORIS DOI:

10.7892/boris.41756

URI:

https://boris.unibe.ch/id/eprint/41756

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