Tularemia in a common marmoset (Callithrix jacchus) diagnosed by 16S rRNA sequencing

Posthaus, Horst; Welle, Monika Maria; Mörner, T; Nicolet, J; Kuhnert, Peter (1998). Tularemia in a common marmoset (Callithrix jacchus) diagnosed by 16S rRNA sequencing. Veterinary microbiology, 61(1-2), pp. 145-150. Elsevier 10.1016/S0378-1135(98)00180-1

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We report a case of tularemia in a common marmoset (Callithrix jacchus) diagnosed by determination of the isolate's 16S ribosomal RNA (rRNA) gene sequence. Pathological examination of the animal revealed a multifocal acute necrotizing hepatitis, interstitial nephritis, splenitis, and lymphangitis of the mandibular, retropharyngeal, and cervical and mesenteric lymph nodes. Moreover, multiple foci of acute necrosis were found in the epithelium of the jejunum and the interstitium of the lung. Bacteriological investigations revealed a septicemia. The isolated infectious agent was uncommon, not routinely diagnosed in our laboratory and therefore difficult to identify by conventional tools in a reasonable time and effort. thus, we decided to perform a genetic analysis based on the 16S rRNA gene sequence. Thereby, an infection with Francisella tularensis, the causative agent of tularemia, was unambiguously diagnosed. This shows the great advantage 16S rRNA gene sequencing has as a general identification approach for unusual or rare isolates.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology

UniBE Contributor:

Posthaus, Horst; Welle, Monika Maria and Kuhnert, Peter

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Peter Kuhnert-Ryser

Date Deposited:

17 Jul 2014 10:14

Last Modified:

17 Jul 2014 10:14

Publisher DOI:

10.1016/S0378-1135(98)00180-1

PubMed ID:

9646473

URI:

https://boris.unibe.ch/id/eprint/42374

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