Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors

Hoppe, Sven; Alini, Mauro; Benneker, Lorin M.; Milz, Stefan; Boileau, Pascal; Zumstein, Matthias A. (2013). Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors. Journal of shoulder and elbow surgery, 22(3), pp. 340-349. Elsevier 10.1016/j.jse.2012.01.016

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BACKGROUND

Bone-to-tendon healing after rotator cuff repairs is mainly impaired by poor tissue quality. The tenocytes of chronic rotator cuff tendon tears are not able to synthesize normal fibrocartilaginous extracellular matrix (ECM). We hypothesized that in the presence of platelet-released growth factors (PRGF), tenocytes from chronically retracted rotator cuff tendons proliferate and synthesize the appropriate ECM proteins.

MATERIALS AND METHODS

Tenocytes from 8 patients with chronic rotator cuff tears were cultured for 4 weeks in 2 different media: standard medium (Iscove's Modified Dulbecco's Media + 10% fetal calf serum + 1% nonessential amino acids + 0.5 μg/mL ascorbic acid) and media with an additional 10% PRGF. Cell proliferation was assessed at 7, 14, 21, and 28 days. Messenger (m)RNA levels of collagens I, II, and X, decorin, biglycan, and aggrecan were analyzed using real time reverse-transcription polymerase chain reaction. Immunocytochemistry was also performed.

RESULTS

The proliferation rate of tenocytes was significantly higher at all time points when cultured with PRGF. At 21 days, the mRNA levels for collagens I, II, and X, decorin, aggrecan, and biglycan were significantly higher in the PRGF group. The mRNA data were confirmed at protein level by immunocytochemistry.

CONCLUSIONS

PRGFs enhance tenocyte proliferation in vitro and promote synthesis of ECM to levels similar to those found with insertion of the normal human rotator cuffs.

CLINICAL RELEVANCE

Biologic augmentation of repaired rotator cuffs with PRGF may enhance the properties of the repair tissue. However, further studies are needed to determine if application of PRGF remains safe and effective in long-term clinical studies.

LEVEL OF EVIDENCE

Basic Science Study, Cell Biology.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
UniBE Contributor:	Hoppe, Sven, Benneker, Lorin Michael, Zumstein, Matthias
Subjects:	600 Technology > 610 Medicine & health
ISSN:	1058-2746
Publisher:	Elsevier
Language:	English
Submitter:	Stephanie Schmutz
Date Deposited:	04 Apr 2014 10:37
Last Modified:	05 Dec 2022 14:28
Publisher DOI:	10.1016/j.jse.2012.01.016
PubMed ID:	22521394
URI:	https://boris.unibe.ch/id/eprint/42403