Bone-Conditioned Medium Inhibits Osteogenic and Adipogenic Differentiation of Mesenchymal Cells In Vitro

Peng, Jianbo; Nemec, Michael; Brolese, Eliane Lucia; Bosshardt, Dieter; Schaller, Benoît; Buser, Daniel; Gruber, Reinhard (2015). Bone-Conditioned Medium Inhibits Osteogenic and Adipogenic Differentiation of Mesenchymal Cells In Vitro. Clinical implant dentistry and related research, 17(5), pp. 938-949. Blackwell 10.1111/cid.12200

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BACKGROUND AND PURPOSE Autografts are used for bone reconstruction in regenerative medicine including oral and maxillofacial surgery. Bone grafts release paracrine signals that can reach mesenchymal cells at defect sites. The impact of the paracrine signals on osteogenic, adipogenic, and chondrogenic differentiation of mesenchymal cells has remained unclear. MATERIAL AND METHODS Osteogenesis, adipogenesis, and chondrogenesis were studied with murine ST2 osteoblast progenitors, 3T3-L1 preadipocytes, and ATDC5 prechondrogenic cells, respectively. Primary periodontal fibroblasts from the gingiva, from the periodontal ligament, and from bone were also included in the analysis. Cells were exposed to bone-conditioned medium (BCM) that was prepared from porcine cortical bone chips. RESULTS BCM inhibited osteogenic and adipogenic differentiation of ST2 and 3T3-L1 cells, respectively, as shown by histological staining and gene expression. No substantial changes in the expression of chondrogenic genes were observed in ATDC5 cells. Primary periodontal fibroblasts also showed a robust decrease in alkaline phosphatase and peroxisome proliferator-activated receptor gamma (PPARγ) expression when exposed to BCM. BCM also increased collagen type 10 expression. Pharmacologic blocking of transforming growth factor (TGF)-β receptor type I kinase with SB431542 and the smad-3 inhibitor SIS3 at least partially reversed the effect of BCM on PPARγ and collagen type 10 expression. In support of BCM having TGF-β activity, the respective target genes were increasingly expressed in periodontal fibroblasts. CONCLUSIONS The present work is a pioneer study on the paracrine activity of bone grafts. The findings suggest that cortical bone chips release soluble signals that can modulate differentiation of mesenchymal cells in vitro at least partially involving TGF-β signaling.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Research

UniBE Contributor:

Peng, Jianbo; Brolese, Eliane Lucia; Bosshardt, Dieter; Schaller, Benoît; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1523-0899

Publisher:

Blackwell

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

01 May 2014 09:04

Last Modified:

11 Apr 2016 10:52

Publisher DOI:

10.1111/cid.12200

PubMed ID:

24461197

Additional Information:

Early View (Online Version of Record published before inclusion in an issue)

Uncontrolled Keywords:

ADAM19, ALPL, COL10, IL-11, MAPK, PPARγ, Sema7a, TGF-β, adipocytes, adipogenic differentiation, autografts, autologous bone grafts, bone regeneration, chondrogenic differentiation, conditioned medium, differentiation, graft consolidation, mesenchymal cells, osteoblasts, osteocytes, osteogenic differentiation, paracrine, periodontal fibroblasts, smad-3, supernatant

BORIS DOI:

10.7892/boris.42654

URI:

https://boris.unibe.ch/id/eprint/42654

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