Cortes-Dericks, Lourdes; Yazd, Ehsan Farashahi; Mowla, Seyed J.; Schmid, Ralph A.; Karoubi, Golnaz (2013). Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis. Anticancer research, 33(12), pp. 5365-5373. International Inst. of Anticancer Research
Full text not available from this repository.BACKGROUND
Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the potential role of Octamer-binding transcription factor-4B (OCT4B) in enhancing sensitivity of lung adenocarcinoma cells to cisplatin.
MATERIALS AND METHODS
RNAi interference was used to examine the role of OCT4B in cisplatin-treated A549 cells. Cells were transfected with OCT4B siRNA prior to a 48-h cisplatin treatment. Propidium iodide (PI) and caspase-3 staining were used to determine cell viability and apoptosis. Cell-cycle analysis was performed to evaluate alterations in phase distribution.
RESULTS
OCT4B suppression in cells increased the number of non-viable, PI(+), and apoptotic, caspase-3(+) cells in the presence and absence of cisplatin treatment. Importantly, cisplatin treatment of OCT4B-suppressed cells resulted in a marked transition of cells from G0/G1 to G2/M phase.
CONCLUSION
Silencing of OCT4B confers sensitivity to cisplatin treatment in A549 cells via cell-cycle regulation, increased proliferation and enhancement of cisplatin-induced apoptosis. OCT4B clearly protects A549 cells from apoptosis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Thoraxchirurgie 04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery |
UniBE Contributor: |
Cortes-Dericks, Lourdes, Schmid, Ralph, Karoubi, Golnaz |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0250-7005 |
Publisher: |
International Inst. of Anticancer Research |
Language: |
English |
Submitter: |
Thomas Michael Marti |
Date Deposited: |
01 May 2014 14:13 |
Last Modified: |
05 Dec 2022 14:29 |
PubMed ID: |
24324071 |
Uncontrolled Keywords: |
A549, OCT4B, apoptosis, chemoresistance, cisplatin |
URI: |
https://boris.unibe.ch/id/eprint/43247 |
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