Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis

Cortes-Dericks, Lourdes; Yazd, Ehsan Farashahi; Mowla, Seyed J.; Schmid, Ralph A.; Karoubi, Golnaz (2013). Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis. Anticancer research, 33(12), pp. 5365-5373. International Inst. of Anticancer Research

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BACKGROUND Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the potential role of Octamer-binding transcription factor-4B (OCT4B) in enhancing sensitivity of lung adenocarcinoma cells to cisplatin. MATERIALS AND METHODS RNAi interference was used to examine the role of OCT4B in cisplatin-treated A549 cells. Cells were transfected with OCT4B siRNA prior to a 48-h cisplatin treatment. Propidium iodide (PI) and caspase-3 staining were used to determine cell viability and apoptosis. Cell-cycle analysis was performed to evaluate alterations in phase distribution. RESULTS OCT4B suppression in cells increased the number of non-viable, PI(+), and apoptotic, caspase-3(+) cells in the presence and absence of cisplatin treatment. Importantly, cisplatin treatment of OCT4B-suppressed cells resulted in a marked transition of cells from G0/G1 to G2/M phase. CONCLUSION Silencing of OCT4B confers sensitivity to cisplatin treatment in A549 cells via cell-cycle regulation, increased proliferation and enhancement of cisplatin-induced apoptosis. OCT4B clearly protects A549 cells from apoptosis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Thoraxchirurgie
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery

UniBE Contributor:

Cortes-Dericks, Lourdes; Schmid, Ralph and Karoubi, Golnaz

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0250-7005

Publisher:

International Inst. of Anticancer Research

Language:

English

Submitter:

Thomas Michael Marti

Date Deposited:

01 May 2014 14:13

Last Modified:

01 May 2014 14:13

PubMed ID:

24324071

Uncontrolled Keywords:

A549, OCT4B, apoptosis, chemoresistance, cisplatin

URI:

https://boris.unibe.ch/id/eprint/43247

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