Stanway, Rebecca R.; Schmuckli-Maurer, Jacqueline; Heussler, Volker (2013). Analysis of liver stage development in and merozoite release from hepatocytes. In: Ménard, Robert (ed.) Malaria, Methods and Protocols. Methods in Molecular Biology: Vol. 923 (pp. 411-427). Humana Press 10.1007/978-1-62703-026-7_29
Full text not available from this repository.Exoerythrocytic Plasmodium parasites infect hepatocytes and develop to huge multinucleated schizonts inside a parasitophorous vacuole. Finally, thousands of merozoites are formed and released into the host cell cytoplasm by complete disintegration of the parasitophorous vacuole membrane. This, in turn, results in death and detachment of the infected hepatocyte, followed by the formation of merosomes. The fast growth of the parasite and host cell detachment are hallmarks of liver stage development and can easily be monitored. Here, we describe how to translate these observations into assays for characterizing parasite development. Additionally, other recently introduced techniques and tools to analyze and manipulate liver stage parasites are also discussed.
Item Type: |
Book Section (Book Chapter) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria |
UniBE Contributor: |
Limenitakis, Rebecca Rachel, Schmuckli, Jacqueline, Heussler, Volker |
Subjects: |
500 Science > 570 Life sciences; biology |
ISSN: |
1064-3745 |
ISBN: |
978-1-62703-025-0 |
Series: |
Methods in Molecular Biology |
Publisher: |
Humana Press |
Language: |
English |
Submitter: |
Jacqueline Schmuckli |
Date Deposited: |
24 Apr 2014 08:53 |
Last Modified: |
02 Mar 2023 23:24 |
Publisher DOI: |
10.1007/978-1-62703-026-7_29 |
PubMed ID: |
22990795 |
URI: |
https://boris.unibe.ch/id/eprint/43443 |
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