Analysis of liver stage development in and merozoite release from hepatocytes

Stanway, Rebecca R.; Schmuckli-Maurer, Jacqueline; Heussler, Volker (2013). Analysis of liver stage development in and merozoite release from hepatocytes. In: Ménard, Robert (ed.) Malaria, Methods and Protocols. Methods in Molecular Biology: Vol. 923 (pp. 411-427). Humana Press 10.1007/978-1-62703-026-7_29

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Exoerythrocytic Plasmodium parasites infect hepatocytes and develop to huge multinucleated schizonts inside a parasitophorous vacuole. Finally, thousands of merozoites are formed and released into the host cell cytoplasm by complete disintegration of the parasitophorous vacuole membrane. This, in turn, results in death and detachment of the infected hepatocyte, followed by the formation of merosomes. The fast growth of the parasite and host cell detachment are hallmarks of liver stage development and can easily be monitored. Here, we describe how to translate these observations into assays for characterizing parasite development. Additionally, other recently introduced techniques and tools to analyze and manipulate liver stage parasites are also discussed.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria

UniBE Contributor:

Limenitakis Stanway, Rebecca; Schmuckli, Jacqueline and Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1064-3745

ISBN:

978-1-62703-025-0

Series:

Methods in Molecular Biology

Publisher:

Humana Press

Language:

English

Submitter:

Jacqueline Schmuckli

Date Deposited:

24 Apr 2014 08:53

Last Modified:

11 May 2016 10:02

Publisher DOI:

10.1007/978-1-62703-026-7_29

PubMed ID:

22990795

URI:

https://boris.unibe.ch/id/eprint/43443

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