Functional Diversification of Dicer-like Proteins and Small RNAs Required for Genome Sculpting.

Sandoval, Pamela Yohanna de Lourdes; Swart, Estienne Carl; Arambasic, Miroslav; Nowacki, Mariusz (2014). Functional Diversification of Dicer-like Proteins and Small RNAs Required for Genome Sculpting. Developmental cell, 28(2), pp. 174-188. Cell Press 10.1016/j.devcel.2013.12.010

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In eukaryotes, small RNAs (sRNAs) have key roles in development, gene expression regulation, and genome integrity maintenance. In ciliates, such as Paramecium, sRNAs form the heart of an epigenetic system that has evolved from core eukaryotic gene silencing components to selectively target DNA for deletion. In Paramecium, somatic genome development from the germline genome accurately eliminates the bulk of typically gene-interrupting, noncoding DNA. We have discovered an sRNA class (internal eliminated sequence [IES] sRNAs [iesRNAs]), arising later during Paramecium development, which originates from and precisely delineates germline DNA (IESs) and complements the initial sRNAs ("scan" RNAs [scnRNAs]) in targeting DNA for elimination. We show that whole-genome duplications have facilitated successive differentiations of Paramecium Dicer-like proteins, leading to cooperation between Dcl2 and Dcl3 to produce scnRNAs and to the production of iesRNAs by Dcl5. These innovations highlight the ability of sRNA systems to acquire capabilities, including those in genome development and integrity.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Ciliate
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Sandoval, Pamela Yohanna de Lourdes; Swart, Estienne Carl; Arambasic, Miroslav and Nowacki, Mariusz

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1534-5807

Publisher:

Cell Press

Language:

English

Submitter:

Mariusz Nowacki

Date Deposited:

23 Jul 2014 16:57

Last Modified:

26 Jun 2016 01:47

Publisher DOI:

10.1016/j.devcel.2013.12.010

BORIS DOI:

10.7892/boris.43499

URI:

https://boris.unibe.ch/id/eprint/43499

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