Walser, Jochen; Caversaccio, Marco; Ferguson, Stephen J (2013). Electrospinning Auricular Shaped Scaffolds for Tissue Engineering. Biomedizinische Technik / Biomedical engineering, 58 Suppl 1(Suppl.) De Gruyter 10.1515/bmt-2013-4203
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Poly(ɛ)caprolactone scaffolds have been electrospun directly into an auricular shaped conductive mould. Bovine chondrocytes were harvested from articular cartilage and seeded onto 16 of the produced scaffolds, which received either an ethanol (group A) or a plasma treatment (group B) for sterilisation before seeding. The seeded scaffolds were cultured for 3 weeks in vitro and analysed
with regard to total DNA and GAG content as well as the expression of AGG, COL1, COL2, MMP3 and MMP13. Rapid cell proliferation and GAG accumulation
was observed until week 2. However, total DNA and GAG content decreased again in week 3. qPCR data shows a slight increase in the expression of anabolic genes and a slight decrease for the catabolic genes, with a significant
difference between the groups A and B only for COL2 and MMP13.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ear, Nose and Throat Disorders (ENT) |
UniBE Contributor: |
Caversaccio, Marco |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0013-5585 |
Publisher: |
De Gruyter |
Language: |
English |
Submitter: |
Martin Kompis |
Date Deposited: |
07 Mar 2014 08:36 |
Last Modified: |
02 May 2023 16:04 |
Publisher DOI: |
10.1515/bmt-2013-4203 |
PubMed ID: |
24042860 |
Uncontrolled Keywords: |
Electrospinning, Tissue Engineering, Auricle |
BORIS DOI: |
10.7892/boris.43560 |
URI: |
https://boris.unibe.ch/id/eprint/43560 |
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