Electrospinning Auricular Shaped Scaffolds for Tissue Engineering

Walser, Jochen; Caversaccio, Marco; Ferguson, Stephen J (2013). Electrospinning Auricular Shaped Scaffolds for Tissue Engineering. Biomedizinische Technik / Biomedical engineering, 58 Suppl 1(Suppl.) De Gruyter 10.1515/bmt-2013-4203

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Poly(ɛ)caprolactone scaffolds have been electrospun directly into an auricular shaped conductive mould. Bovine chondrocytes were harvested from articular cartilage and seeded onto 16 of the produced scaffolds, which received either an ethanol (group A) or a plasma treatment (group B) for sterilisation before seeding. The seeded scaffolds were cultured for 3 weeks in vitro and analysed with regard to total DNA and GAG content as well as the expression of AGG, COL1, COL2, MMP3 and MMP13. Rapid cell proliferation and GAG accumulation was observed until week 2. However, total DNA and GAG content decreased again in week 3. qPCR data shows a slight increase in the expression of anabolic genes and a slight decrease for the catabolic genes, with a significant difference between the groups A and B only for COL2 and MMP13.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ear, Nose and Throat Disorders (ENT)

UniBE Contributor:

Caversaccio, Marco

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0013-5585

Publisher:

De Gruyter

Language:

English

Submitter:

Martin Kompis

Date Deposited:

07 Mar 2014 08:36

Last Modified:

24 Oct 2019 05:24

Publisher DOI:

10.1515/bmt-2013-4203

PubMed ID:

24042860

Uncontrolled Keywords:

Electrospinning, Tissue Engineering, Auricle

BORIS DOI:

10.7892/boris.43560

URI:

https://boris.unibe.ch/id/eprint/43560

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