Unilateral renal denervation and the renal kallikrein-bradykinin system in the rat

Bohlender, J.; Nussberger, J.; Amstutz, C.; Birkhäuser, F.; Thalmann, G. N.; Imboden, H. (2013). Unilateral renal denervation and the renal kallikrein-bradykinin system in the rat. Annales de cardiologie et d'angéiologie, 62(3), pp. 144-148. Elsevier Masson 10.1016/j.ancard.2013.04.014

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Aim The antihypertensive effect of renal denervation in hypertensive patients is partially explained by increased tubular natriuresis. To study the possible contribution of the kallikrein-kinin system (KKS) to this natriuretic effect in rats, we measured kallikrein activity (KA) and bradykinin concentrations (BK) in plasma and tissues. Methods To measure KA, we adapted and validated an enzymatic assay that cleaves para-nitroaniline (pNA) from the tripeptide H-D-Pro-Phe-Arg-pNA. The coefficients of variation (CV) within- and between-assays were less than 8% for plasma and tissue KA (plasma n = 6 and 13; tissue n = 4). Linear results for serially diluted samples confirmed the assay specificity. Tissue BK determinations were based on an established assay for plasma BK: tissue was homogenized and kinins extracted in ethanol, and BK was isolated by high-performance (HPLC) liquid chromatography and quantitated by radioimmunassay. Within- and between-assay CV for plasma BK were 18% (n = 8 and n = 35, respectively) and for BK in various tissues less than 16% (n = 5-8). Results In male Wistar rats (n = 3), plasma BK was 8.2 ± 6.6 fmol/mL (mean ± SD), and tissue BK (fmol/g) in 14 tested organs varied between brain (14 ± 3) and submaxillary gland (521 ± 315). Six days after left-sided unilateral renal denervation, left renal tissue BK (89 ± 9) was not different from right renal BK (75 ± 23). Similarly, KA was comparable in the two kidneys (left 18.0 ± 1.5, right 15.8 ± 1.4 μkat/g). Conclusion Any possible effect of unilateral renal denervation on the kidney's KKS would have to be bilateral.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Urology

UniBE Contributor:

Bohlender, Jürgen; Birkhäuser, Frédéric; Thalmann, George and Imboden, Hans

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0003-3928

Publisher:

Elsevier Masson

Language:

French

Submitter:

Katharina Morgenegg

Date Deposited:

23 Apr 2014 11:05

Last Modified:

10 Nov 2017 10:58

Publisher DOI:

10.1016/j.ancard.2013.04.014

BORIS DOI:

10.7892/boris.43737

URI:

https://boris.unibe.ch/id/eprint/43737

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