Comparison of paired serum and lithium heparin plasma samples for the measurement of serum amyloid A in horses using an automated turbidimetric immunoassay

Howard, J.; Graubner, Claudia (2014). Comparison of paired serum and lithium heparin plasma samples for the measurement of serum amyloid A in horses using an automated turbidimetric immunoassay. Veterinary journal, 199(3), pp. 457-460. Elsevier 10.1016/j.tvjl.2013.12.007

[img] Text
1-s2.0-S1090023313006278-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (681kB) | Request a copy

This study aimed to evaluate whether equine serum amyloid A (SAA) concentrations could be reliably measured in plasma with a turbidimetric immunoassay previously validated for equine SAA concentrations in serum. Paired serum and lithium-heparin samples obtained from 40 horses were evaluated. No difference was found in SAA concentrations between serum and plasma using a paired t test (P=0.48). The correlation between paired samples was 0.97 (Spearman's rank P<0.0001; 95% confidence interval 0.95-0.99). Passing-Bablok regression analyses revealed no differences between paired samples. Bland-Altman plots revealed a positive bias in plasma compared to serum but the difference was not considered clinically significant. The results indicate that lithium-heparin plasma samples are suitable for measurement of equine SAA using this method. Use of either serum or plasma allows for greater flexibility when it comes to sample collection although care should be taken when comparing data between measurements from different sample types.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Equine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Central Clinical Laboratory

UniBE Contributor:

Howard, Judith and Graubner, Claudia

Subjects:

600 Technology > 630 Agriculture

ISSN:

1090-0233

Publisher:

Elsevier

Language:

English

Submitter:

Susanne Portner

Date Deposited:

22 Aug 2014 15:17

Last Modified:

14 Oct 2015 11:14

Publisher DOI:

10.1016/j.tvjl.2013.12.007

PubMed ID:

24412695

BORIS DOI:

10.7892/boris.43761

URI:

https://boris.unibe.ch/id/eprint/43761

Actions (login required)

Edit item Edit item
Provide Feedback