Cattle immunized against the pathogenic L-α-glycerol-3-phosphate oxidase of Mycoplasma mycoides subs. mycoides fail to generate neutralizing antibodies and succumb to disease on challenge

Mulongo, Musa M.; Frey, Joachim; Smith, Ken; Schnier, Christian; Wesonga, Hezron; Naessens, Jan; McKeever, Declan (2013). Cattle immunized against the pathogenic L-α-glycerol-3-phosphate oxidase of Mycoplasma mycoides subs. mycoides fail to generate neutralizing antibodies and succumb to disease on challenge. Vaccine, 31(44), pp. 5020-5025. Elsevier 10.1016/j.vaccine.2013.08.100

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The membrane-associated enzyme L-α-glycerol-3-phosphate oxidase (GlpO) of Mycoplasma mycoides subs. mycoides (Mmm), the causal agent of contagious bovine pleuropneumonia (CBPP) has been identified as a virulence factor responsible for the release of toxic by-products such as H2O2 that mediate host cell injury. Since CBPP pathogenesis is based on host inflammatory reactions, we have determined the capacity of recombinant GlpO to generate in vivo protective responses against challenge in immunized cattle. We also investigated whether sera raised against recombinant GlpO in cattle and mice inhibit production of H2O2 by Mmm. Immunization of cattle with recombinant GlpO did not protect against challenge with a virulent strain of Mmm. Further, although both murine and bovine antisera raised against recombinant GlpO detected recombinant and native forms of GlpO in immunoblot assays with similar titres, only murine antibodies could neutralize GlpO enzymatic function. The data raise the possibility that Mmm has adapted to evade potential detrimental antibody responses in its definitive host.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Frey, Joachim

Subjects:

600 Technology > 630 Agriculture

ISSN:

0264-410X

Publisher:

Elsevier

Language:

English

Submitter:

Susanne Portner

Date Deposited:

22 Aug 2014 13:28

Last Modified:

11 Feb 2015 13:33

Publisher DOI:

10.1016/j.vaccine.2013.08.100

PubMed ID:

24035434

Uncontrolled Keywords:

Mycoplasma, Antibody, Vaccination, Cattle, Evolution, l-α-glycerol-3-phosphate oxidase

BORIS DOI:

10.7892/boris.43814

URI:

https://boris.unibe.ch/id/eprint/43814

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