Clustering subspecies of Aeromonas salmonicida using IS630 typing.

Studer, Nicole; Frey, Joachim; Vanden Bergh, Philippe (2013). Clustering subspecies of Aeromonas salmonicida using IS630 typing. BMC microbiology, 13(36), p. 36. BioMed Central 10.1186/1471-2180-13-36

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BACKGROUND The insertion element IS630 found in Aeromonas salmonicida belongs to the IS630-Tc1-mariner superfamily of transposons. It is present in multiple copies and represents approximately half of the IS present in the genome of A. salmonicida subsp. salmonicida A449. RESULTS By using High Copy Number IS630 Restriction Fragment Length Polymorphism (HCN-IS630-RFLP), strains of various subspecies of Aeromonas salmonicida showed conserved or clustering patterns, thus allowing their differentiation from each other. Fingerprints of A. salmonicida subsp. salmonicida showed the highest homogeneity while 'atypical' A. salmonicida strains were more heterogeneous. IS630 typing also differentiated A. salmonicida from other Aeromonas species. The copy number of IS630 in Aeromonas salmonicida ranges from 8 to 35 and is much lower in other Aeromonas species. CONCLUSIONS HCN-IS630-RFLP is a powerful tool for subtyping of A. salmonicida. The high stability of IS630 insertions in A. salmonicida subsp. salmonicida indicates that it might have played a role in pathoadaptation of A. salmonicida which has reached an optimal configuration in the highly virulent and specific fish pathogen A. salmonicida subsp. salmonicida.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Studer, Nicole and Frey, Joachim

Subjects:

600 Technology > 630 Agriculture

ISSN:

1471-2180

Publisher:

BioMed Central

Language:

English

Submitter:

Susanne Portner

Date Deposited:

18 Jul 2014 12:17

Last Modified:

26 Jun 2016 01:47

Publisher DOI:

10.1186/1471-2180-13-36

PubMed ID:

23406017

BORIS DOI:

10.7892/boris.43832

URI:

https://boris.unibe.ch/id/eprint/43832

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