Expression of thymic stromal lymphopoietin in canine atopic dermatitis

Klukowska-Rötzler, Jolanta; Chervet, Ludovic; Müller, Eliane Jasmine; Roosje, Petra; Marti, Eliane Isabelle; Janda, Jozef (2013). Expression of thymic stromal lymphopoietin in canine atopic dermatitis. Veterinary dermatology, 24(1), 54-e14. Blackwell Science 10.1111/j.1365-3164.2012.01096.x

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BACKGROUND In humans, thymic stromal lymphopoietin (TSLP) plays a central role in the development of allergic inflammation, such as atopic dermatitis (AD), but it is unknown whether it is involved in the pathogenesis of canine AD (CAD). HYPOTHESIS/OBJECTIVES Our aim was to characterize canine TSLP and to assess its expression in CAD. METHODS Canine TSLP was identified based on sequence homology with human TSLP and the complementary DNA (cDNA) cloned by RT-PCR. Real-time quantitative RT-PCR was established to assess the expression of canine TSLP in cultured canine keratinocytes and in skin biopsy specimens from lesional and nonlesional skin of 12 dogs with CAD and eight healthy control dogs. RESULTS Partial canine TSLP cDNA was cloned and characterized. It contained four exons that shared 70 and 73% nucleotide identity with human and equine TSLP, respectively, encoding the signal peptide and full-length secreted protein. We found significantly increased TSLP expression in lesional and nonlesional skin of dogs with CAD compared with healthy control dogs (P < 0.05), whereas no difference was measured between lesional and nonlesional samples. In cultured primary canine keratinocytes, we found increased TSLP expression after stimulation with house dust mite allergen extract or Toll-like receptor ligands lipopolysaccharide and poly I:C. CONCLUSIONS AND CLINICAL IMPORTANCE Increased TSLP expression in the skin of dogs with CAD supports an involvement of TSLP in the pathogenesis of CAD similar to that in humans. Further studies should elucidate the function and therapeutic potential of TSLP in CAD.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Dermatology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Klukowska, Jolanta; Müller, Eliane Jasmine; Roosje, Petra; Marti, Eliane Isabelle and Janda, Jozef

Subjects:

600 Technology > 630 Agriculture

ISSN:

0959-4493

Publisher:

Blackwell Science

Language:

English

Submitter:

Susanne Portner

Date Deposited:

28 Jul 2014 15:35

Last Modified:

14 Oct 2015 11:36

Publisher DOI:

10.1111/j.1365-3164.2012.01096.x

PubMed ID:

23331680

URI:

https://boris.unibe.ch/id/eprint/44051

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