Analysis of protein expression in zebrafish during gonad differentiation by targeted proteomics

Groh, Ksenia J; Schönenberger, René; Eggen, Rik I L; Segner, Helmut; Suter, Marc J-F (2013). Analysis of protein expression in zebrafish during gonad differentiation by targeted proteomics. General and comparative endocrinology, 193, pp. 210-220. Elsevier 10.1016/j.ygcen.2013.07.020

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The molecular mechanisms governing sex determination and differentiation in the zebrafish (Danio rerio) are not fully understood. To gain more insights into the function of specific genes in these complex processes, the expression of multiple candidates needs to be assessed, preferably on the protein level. Here, we developed a targeted proteomics method based on selected reaction monitoring (SRM) to study the candidate sex-related proteins in zebrafish which were selected based on a global proteomics analysis of adult gonads and representational difference analysis of male and female DNA, as well as on published information on zebrafish and other vertebrates. We employed the developed SRM protocols to acquire time-resolved protein expression profiles during the gonad differentiation period in vas::EGFP transgenic zebrafish. Evidence on protein expression was obtained for the first time for several candidate genes previously studied only on the mRNA level or suggested by bioinformatic predictions. Tuba1b (tubulin alpha 1b), initially included in the study as one of the potential housekeeping proteins, was found to be preferentially expressed in the adult testis with nearly absent expression in the ovary. The revealed changes in protein expression patterns associated with gonad differentiation suggest that several of the examined proteins, especially Ilf2 and Ilf3 (interleukin enhancer-binding factors 2 and 3), Raldh3 (retinaldehyde dehydrogenase type 3), Zgc:195027 (low density lipoprotein-related receptor protein 3) and Sept5a (septin 5a), may play a specific role in the sexual differentiation in zebrafish.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

0016-6480

Publisher:

Elsevier

Language:

English

Submitter:

Susanne Portner

Date Deposited:

22 Aug 2014 11:43

Last Modified:

14 Oct 2015 11:15

Publisher DOI:

10.1016/j.ygcen.2013.07.020

PubMed ID:

23968773

Uncontrolled Keywords:

Zebrafish, Sexual differentiation, Development, Mass spectrometry, Selected reaction monitoring (SRM), Targeted proteomics

BORIS DOI:

10.7892/boris.44293

URI:

https://boris.unibe.ch/id/eprint/44293

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